This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, S.
Right arrow Articles by Hughes, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, S.
Right arrow Articles by Hughes, J. B.
Agricola
Right arrow Articles by Huang, S.
Right arrow Articles by Hughes, J. B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2000, p. 1474-1478, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

2,4,6-Trinitrotoluene Reduction by Carbon Monoxide Dehydrogenase from Clostridium thermoaceticum

Shouqin Huang,1 Paul A. Lindahl,2 Chuanyue Wang,3 George N. Bennett,1,* Frederick B. Rudolph,1 and Joseph B. Hughes3

Department of Biochemistry and Cell Biology and the Institute of Biosciences and Bioengineering1 and Department of Environmental Science and Engineering,3 Rice University, Houston, Texas 77005-1892, and The Departments of Chemistry and of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 778432

Received 24 September 1999/Accepted 19 January 2000

Purified CO dehydrogenase (CODH) from Clostridium thermoaceticum catalyzed the transformation of 2,4,6-trinitrotoluene (TNT). The intermediates and reduced products of TNT transformation were separated and appear to be identical to the compounds formed by C. acetobutylicum, namely, 2-hydroxylamino-4,6-dinitrotoluene (2HA46DNT), 4-hydroxylamino-2,6-dinitrotoluene (4HA26DNT), 2,4-dihydroxylamino-6-nitrotoluene (24DHANT), and the Bamberger rearrangement product of 2,4-dihydroxylamino-6-nitrotoluene. In the presence of saturating CO, CODH catalyzed the conversion of TNT to two monohydroxylamino derivatives (2HA46DNT and 4HA26DNT), with 4HA26DNT as the dominant isomer. These derivatives were then converted to 24DHANT, which slowly converted to the Bamberger rearrangement product. Apparent Km and kcat values of TNT reduction were 165 ± 43 µM for TNT and 400 ± 94 s-1, respectively. Cyanide, an inhibitor for the CO/CO2 oxidation/reduction activity of CODH, inhibited the TNT degradation activity of CODH.

* Corresponding author. Mailing address: Department of Biochemistry and Cell Biology, MS-140, Rice University, 6100 Main St., Houston, TX 77005-1892. Phone: (713) 348-4920. Fax: (713) 348-5154. E-mail: gbennett{at}bioc.rice.edu.

Applied and Environmental Microbiology, April 2000, p. 1474-1478, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ziganshin, A. M., Gerlach, R., Borch, T., Naumov, A. V., Naumova, R. P. (2007). Production of Eight Different Hydride Complexes and Nitrite Release from 2,4,6-Trinitrotoluene by Yarrowia lipolytica. Appl. Environ. Microbiol. 73: 7898-7905 [Abstract] [Full Text]  
  • Stenuit, B., Eyers, L., Rozenberg, R., Habib-Jiwan, J.-L., Agathos, S. N. (2006). Aerobic Growth of Escherichia coli with 2,4,6-Trinitrotoluene (TNT) as the Sole Nitrogen Source and Evidence of TNT Denitration by Whole Cells and Cell-Free Extracts. Appl. Environ. Microbiol. 72: 7945-7948 [Abstract] [Full Text]  
  • Watrous, M. M., Clark, S., Kutty, R., Huang, S., Rudolph, F. B., Hughes, J. B., Bennett, G. N. (2003). 2,4,6-Trinitrotoluene Reduction by an Fe-Only Hydrogenase in Clostridium acetobutylicum. Appl. Environ. Microbiol. 69: 1542-1547 [Abstract] [Full Text]  
  • Riefler, R. G., Smets, B. F. (2002). NAD(P)H:Flavin Mononucleotide Oxidoreductase Inactivation during 2,4,6-Trinitrotoluene Reduction. Appl. Environ. Microbiol. 68: 1690-1696 [Abstract] [Full Text]  
  • Esteve-Nunez, A., Caballero, A., Ramos, J. L. (2001). Biological Degradation of 2,4,6-Trinitrotoluene. Microbiol. Mol. Biol. Rev. 65: 335-352 [Abstract] [Full Text]  
  • Pak, J. W., Knoke, K. L., Noguera, D. R., Fox, B. G., Chambliss, G. H. (2000). Transformation of 2,4,6-Trinitrotoluene by Purified Xenobiotic Reductase B from Pseudomonas fluorescens I-C. Appl. Environ. Microbiol. 66: 4742-4750 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»