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Applied and Environmental Microbiology, April 2000, p. 1532-1537, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sequencing and Expression of Additional Xylanase Genes from the Hyperthermophile Thermotoga maritima FjSS3B.1

Rosalind A. Reeves,1 Moreland D. Gibbs,1 Daniel D. Morris,1 Katherine R. Griffiths,1 David J. Saul,2 and Peter L. Bergquist1,3,*

Department of Biological Sciences, Macquarie University, Sydney New South Wales, Australia,1 and Centre for Gene Technology, University of Auckland,2 and Department of Molecular Medicine, University of Auckland Medical School,3 Auckland, New Zealand

Received 19 October 1999/Accepted 10 January 2000

Two genes, xynB and xynC, coding for xylanases were isolated from Thermotoga maritima FjSS3B.1 by a genomic-walking-PCR technique. Sequencing of the genes showed that they encode multidomain family 10 xylanases. Only XynB exhibited activity against xylan substrates. The temperature optimum (87°C) and pH optimum (pH 6.5) of XynB are different from the previously reported xylanase, XynA (also a family 10 enzyme), from this organism. The catalytic domain expressed without other domains has a lower temperature optimum, is less thermostable, and has optimal activity at pH 6.5. Despite having a high level of sequence similarity to xynB, xynC appears to be nonfunctional since its encoded protein did not show significant activity on xylan substrates.

* Corresponding author. Mailing address: Research Office, Macquarie University, Sydney, NSW 2109, Australia. Phone: 61-2-9850-8614. Fax: 61-2-9850-8799. E-mail: peter.bergquist{at}mq.edu.au.

Applied and Environmental Microbiology, April 2000, p. 1532-1537, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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