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Applied and Environmental Microbiology, April 2000, p. 1544-1552, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Diversity of Salmonella Strains Isolated from the Aquatic Environment as Determined by Serotyping and Amplification of the Ribosomal DNA Spacer Regions

Julia Baudart,1 Karine Lemarchand,1 Anne Brisabois,2 and Philippe Lebaron1,*

Observatoire Océanologique, Université Pierre et Marie Curie, CNRS UMR 7621, Institut National des Sciences de l'Univers, F-66651 Banyuls-sur-Mer Cedex,1 and Agence Française de Sécurité Sanitaire des Aliments (AFSSA), 75015 Paris Cedex,2 France

Received 3 November 1999/Accepted 27 January 2000

Salmonella species are pathogenic bacteria often detected in sewage, freshwater, marine coastal water, and groundwater. Salmonella spp. can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. However, the diversity of species found in the natural environment remains unknown. The aim of this study was to investigate the diversity of Salmonella strains isolated from different natural aquatic systems within a Mediterranean coastal watershed (river, wastewater, and marine coastal areas). A total of 574 strains isolated from these natural environments were identified by both conventional serotyping and the ribosomal spacer-heteroduplex polymorphism (RS-HP) method (M. A. Jensen and N. Straus, PCR Methods Appl. 3:186-194, 1993). More than 40 different serotypes were found, and some serotypes probably mobilized from widespread animal-rearing activities were detected only during storm events. These serotypes may be good indicators of specific contamination sources. Furthermore, the RS-HP method based on the PCR amplification of the intergenic spacer region between the 16S and 23S rRNA genes can produce amplicon profiles allowing the discrimination of species at both serotype and intraserotype levels. This method represents a powerful tool that could be used for rapid typing of Salmonella isolates.

* Corresponding author. Mailing address: Observatoire Océanologique, Université Pierre et Marie Curie, CNRS UMR 7621, Institut National des Sciences de l'Univers, BP44, F-66651 Banyuls-sur-Mer Cedex, France. Phone: (33) 4-6888-7353. Fax: (33) 4-6888-7395. E-mail: lebaron{at}arago.obs-banyuls.fr.

Applied and Environmental Microbiology, April 2000, p. 1544-1552, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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