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Applied and Environmental Microbiology, April 2000, p. 1572-1579, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Compatible-Solute-Supported Periplasmic Expression of Functional Recombinant Proteins under Stress Conditions

S. Barth,1,* M. Huhn,1 B. Matthey,1 A. Klimka,1 E. A. Galinski,2 and A. Engert1

Department I of Internal Medicine, University of Cologne, Cologne,1 and Institute of Biochemistry, University of Münster, Münster,2 Germany

Received 12 October 1999/Accepted 16 January 2000

The standard method of producing recombinant proteins such as immunotoxins (rITs) in large quantities is to transform gram-negative bacteria and subsequently recover the desired protein from inclusion bodies by intensive de- and renaturing procedures. The major disadvantage of this technique is the low yield of active protein. Here we report the development of a novel strategy for the expression of functional rIT directed to the periplasmic space of Escherichia coli. rITs were recovered by freeze-thawing of pellets from shaking cultures of bacteria grown under osmotic stress (4% NaCl plus 0.5 M sorbitol) in the presence of compatible solutes. Compatible solutes, such as glycine betaine and hydroxyectoine, are low-molecular-weight osmolytes that occur naturally in halophilic bacteria and are known to protect proteins at high salt concentrations. Adding 10 mM glycine betaine for the cultivation of E. coli under osmotic stress not only allowed the bacteria to grow under these otherwise inhibitory conditions but also produced a periplasmic microenvironment for the generation of high concentrations of correctly folded rITs. Protein purified by combinations of metal ion affinity and size exclusion chromatography was substantially stabilized in the presence of 1 M hydroxyecotine after several rounds of freeze-thawing, even at very low protein concentrations. The binding properties and cytotoxic potency of the rITs were confirmed by competitive experiments. This novel compatible-solute-guided expression and purification strategy might also be applicable for high-yield periplasmic production of recombinant proteins in different expression systems.

* Corresponding author. Mailing address: Medizinische Klinik I der Universität zu Köln, Labor für Immuntherapie, Joseph-Stelzmann-Str. 9, D-50931 Köln, Germany. Phone: 49 (0) 221 478-3593. Fax: 49 (0) 221 478-6383. E-mail: stefan.barth{at}uni-koeln.de.

Applied and Environmental Microbiology, April 2000, p. 1572-1579, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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