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Applied and Environmental Microbiology, April 2000, p. 1646-1653, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development and Evaluation of a Model Predicting the Survival of Escherichia coli O157:H7 NCTC 12900 in Homemade Eggplant Salad at Various Temperatures, pHs, and Oregano Essential Oil Concentrations

Panagiotis N. Skandamis and George-John E. Nychas*

Department of Food Science and Technology, Laboratory of Microbiology and Biotechnology of Foods, Agricultural University of Athens, Athens 11855, Greece

Received 9 August 1999/Accepted 27 January 2000

Homemade eggplant salad, a traditional Greek appetizer, was inoculated with Escherichia coli O157:H7 NCTC 12900 supplemented with different concentrations of oregano essential oil (0.0, 0.7, 1.4, and 2.1% [vol/wt]) and stored at different temperatures (0, 5, 10, and 15°C). The product's pH was adjusted to 4.0, 4.5, or 5.0 with lemon juice. For each combination of the environmental factors, the bacterial counts were modeled, using the Baranyi model, as a function of time to estimate the kinetic parameters of the pathogen. A reduction of more than 1 log unit in E. coli O157:H7 counts was observed in all cases, and the death rate depended on the pH, the storage temperature, and the essential oil concentration. Separate quadratic models were developed with natural logarithms of the shoulder period and death rate as estimated by the growth model, as a function of temperature, pH, and oregano essential oil concentrations. These were further used to predict the population of E. coli O157:H7 NCTC 12900 from other inoculated eggplant salads at random conditions of temperature, pH, and oregano oil concentration. The predicted values were compared with viable-count measurements for validation.

* Corresponding author. Mailing address: Department of Food Science and Technology, Laboratory of Microbiology and Biotechnology of Foods, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece. Phone and fax: 30-1-5294693. E-mail: gjn{at}auadec.aua.gr.

Applied and Environmental Microbiology, April 2000, p. 1646-1653, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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