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Applied and Environmental Microbiology, April 2000, p. 1662-1667, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Viable-but-Nonculturable State Induced by Abiotic Stress in the Biocontrol Agent Pseudomonas fluorescens CHA0 Does Not Promote Strain Persistence in Soil

Fabio Mascher,1 Carsten Hase,1 Yvan Moënne-Loccoz,1,2 and Geneviève Défago1,*

Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland,1 and UMR CNRS Ecologie Microbienne du Sol, Université Claude Bernard (Lyon 1), F-69622 Villeurbanne, France2

Received 11 October 1999/Accepted 17 January 2000

The effects of oxygen limitation, low redox potential, and high NaCl stress for 7 days in vitro on the rifampin-resistant biocontrol inoculant Pseudomonas fluorescens CHA0-Rif and its subsequent persistence in natural soil for 54 days were investigated. Throughout the experiment, the strain was monitored using total cell counts (immunofluorescence microscopy), Kogure's direct viable counts, and colony counts (on rifampin-containing plates). Under in vitro conditions, viable-but-nonculturable (VBNC) cells of CHA0-Rif were obtained when the strain was exposed to a combination of low redox potential (230 mV) and oxygen limitation. This mimics a situation observed in the field, where VBNC cells of the strain were found in the waterlogged soil layer above the plow pan. Here, VBNC cells were also observed in vitro when CHA0-Rif was subjected to high NaCl levels (i.e., NaCl at 1.5 M but not 0.7 M). In all treatments, cell numbers remained close to the inoculum level for the first 12 days after inoculation of soil, regardless of the cell enumeration method used, but decreased afterwards. At the last two samplings in soil, VBNC cells of CHA0-Rif were found in all treatments except the one in which log-phase cells had been used. In the two treatments that generated high numbers of VBNC cells in vitro, VBNC cells did not display enhanced persistence compared with culturable cells once introduced into soil, which suggests that this VBNC state did not represent a physiological strategy to improve survival under adverse conditions.

* Corresponding author. Mailing address: Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland. Phone: 41 1 632 38 69. Fax: 41 1 632 11 08. E-mail: genevieve.defago{at}ipw.agrl.ethz.ch.

Applied and Environmental Microbiology, April 2000, p. 1662-1667, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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