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Applied and Environmental Microbiology, April 2000, p. 1711-1714, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expression of the Antifeeding Gene anfA1 in Serratia entomophila Requires RpoS

Stephen R. Giddens,1 Antonio Tormo,2 and H. Khris Mahanty1,*

Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand,1 and Departamento de Bioquimica y Biologia Molecular I, Universidad Complutense de Madrid, Madrid, Spain2

Received 5 October 1999/Accepted 31 December 1999

The rpoS gene of Serratia entomophila BC4B was cloned and used to create rpoS-mutant strain BC4BRS. Larvae of the New Zealand grass grub Costelytra zealandica infected with BC4BRS became amber colored but continued to feed, albeit to a lesser extent than infected larvae. Subsequently, we found that expression of the antifeeding gene anfA1 in trans was substantially reduced in BC4BRS relative to that in the parental strain BC4B. Our data show that a functional rpoS gene is vital for full expression of anfA1 and for development of the antifeeding component of amber disease.

* Corresponding author. Mailing address: Department of Plant and Microbial Sciences, University of Canterbury, Private Bag, Christchurch, New Zealand. Phone: 64-3-364-2730. Fax: 64-3-3642083. E-mail: k.mahanty{at}botn.canterbury.ac.nz.

Applied and Environmental Microbiology, April 2000, p. 1711-1714, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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