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Applied and Environmental Microbiology, April 2000, p. 1730-1733, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effect of Copper Speciation on Whole-Cell Soluble Methane Monooxygenase Activity in Methylosinus trichosporium OB3b

John D. Morton, Kim F. Hayes, and Jeremy D. Semrau*

Environmental and Water Resources Engineering, Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2125

Received 1 November 1999/Accepted 21 January 2000

Soluble methane monooxygenase (sMMO) activity in Methylosinus trichosporium OB3b was found to be more strongly affected as copper-to-biomass ratios changed in a newly developed medium, M2M, which uses pyrophosphate for metal chelation, than in nitrate mineral salts (NMS), which uses EDTA. When M2M medium was amended with EDTA, sMMO activity was similar to that in NMS medium, indicating that EDTA-bound copper had lower bioavailability than pyrophosphate-bound copper. EDTA did not limit the association of copper with the cells; rather, copper was sequestered in a form which did not affect sMMO activity.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, The University of Michigan, 1351 Beal Ave., 181 EWRE Building, Ann Arbor, MI 48109-2125. Phone: (734) 764-6487. Fax: (734) 763-2275. E-mail: jsemrau{at}engin.umich.edu.

Applied and Environmental Microbiology, April 2000, p. 1730-1733, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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