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Applied and Environmental Microbiology, April 2000, p. 1744-1748, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Method for Rapid Purification of Class IIa Bacteriocins and Comparison of Their Activities

D. Guyonnet,1 C. Fremaux,2 Y. Cenatiempo,3 and J. M. Berjeaud3,*

IFR-CNRS, FR 59, "Communication Cellulaire,1" and Laboratoire de Biologie Moléculaire, CNRS ESA 6031, IBMIG, 86022 Poitiers,3 and RHODIA-FOOD, 86220 Dangé Saint-Romain,2 France

Received 2 September 1999/Accepted 6 January 2000

A three-step method was developed for the purification of mesentericin Y105 (60% yield) from the culture supernatant of Leuconostoc mesenteroides Y105. The same procedure was successfully applied to the purification of five other anti-Listeria bacteriocins identified by mass spectrometry. Specific activities of the purified bacteriocins were compared.

* Corresponding author. Mailing address: Lab. de Biologie Moléculaire, IBMIG, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France. Phone: (33) 5 49 45 40 06. Fax: (33) 5 49 45 35 03. E-mail: jean-marc.berjeaud{at}campus.univ-poitiers.fr.

Applied and Environmental Microbiology, April 2000, p. 1744-1748, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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