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Applied and Environmental Microbiology, April 2000, p. 1759-1763, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pathogenic Role of SEF14, SEF17, and SEF21 Fimbriae in Salmonella enterica Serovar Enteritidis Infection of Chickens

Gireesh Rajashekara,1,dagger Shirin Munir,1 Mikhail F. Alexeyev,2 David A. Halvorson,1 Carol L. Wells,3 and Kakambi V. Nagaraja1,*

Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota 551081; Department of Microbiology and Immunology, University of South Alabama, Mobile, Alabama 366882; and Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota 554553

Received 15 June 1999/Accepted 3 January 2000

Very little is known about the contribution of surface appendages of Salmonella enterica serovar Enteritidis to pathogenesis in chickens. This study was designed to clarify the role of SEF14, SEF17, and SEF21 fimbriae in serovar Enteritidis pathogenesis. Stable, single, defined sefA (SEF14), agfA (SEF17), and fimA (SEF21) insertionally inactivated fimbrial gene mutants of serovar Enteritidis were constructed. All mutant strains invaded Caco-2 and HT-29 enterocytes at levels similar to that of the wild type. Both mutant and wild-type strains were ingested equally well by chicken macrophage cell lines HD11 and MQ-NCSU. There were no significant differences in the abilities of these strains to colonize chicken ceca. The SEF14- strain was isolated in lower numbers from the livers of infected chickens and was cleared from the spleens faster than other strains. No significant differences in fecal shedding of these strains were observed.

* Corresponding author. Mailing address: Department of Veterinary PathoBiology, 1971 Commonwealth Ave., University of Minnesota, St. Paul, MN 55108. Phone: (612) 625-9704. Fax: (612) 625-5203. E-mail: nagar001{at}maroon.tc.umn.edu.

dagger Present address: Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322.

Applied and Environmental Microbiology, April 2000, p. 1759-1763, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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