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Applied and Environmental Microbiology, May 2000, p. 2079-2084, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Inhibition of Luminescence and Virulence in the Black Tiger Prawn (Penaeus monodon) Pathogen Vibrio harveyi by Intercellular Signal Antagonists

Michael Manefield,1,* Lachlan Harris,2,* Scott A. Rice,1,3 Rocky de Nys,3,4 and Staffan Kjelleberg1,3

Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville,2 and School of Microbiology and Immunology,1 Centre for Marine Biofouling and Bio-Innovation3 and School of Biological Sciences,4 University of New South Wales, Sydney, Australia

Received 22 October 1999/Accepted 17 January 2000

Expression of luminescence in the Penaeus monodon pathogen Vibrio harveyi is regulated by an intercellular quorum sensing mechanism involving the synthesis and detection of two signaling molecules, one of which is N-hydroxy butanoyl-L-homoserine lactone and the other of which is uncharacterized. Indirect evidence has suggested that virulence, associated with a toxic extracellular protein, and luminescence in V. harveyi are coregulated. In this study the effects of an acylated homoserine lactone antagonist produced by the marine alga Delisea pulchra on luminescence and toxin production in a virulent strain of V. harveyi were analyzed. Luminescence and toxin production were both inhibited by the signal antagonist at concentrations that had no impact on growth. Toxin production was found to be prematurely induced in V. harveyi cultures incubated in a 10% conditioned medium. Additionally, a significant reduction in the toxicity of concentrated supernatant extracts from V. harveyi cultures incubated in the presence of the signal antagonist, as measured by in vivo toxicity assays in mice and prawns, was observed. These results suggest that intercellular signaling antagonists have potential utility in the control of V. harveyi prawn infections.

* Corresponding author. Mailing address for Michael Manefield: School of Microbiology and Immunology, University of New South Wales, Sydney 2052, Australia. Phone: 02 93852102. Fax: 02 93851591. E-mail: m.manefield{at}unsw.edu.au. Mailing address for Lachlan Harris: Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville, Australia 4811. Phone: 08 40613706. Fax: 08 40613566. E-mail: lachlanharris{at}internetnorth.com.au.

Applied and Environmental Microbiology, May 2000, p. 2079-2084, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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