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Applied and Environmental Microbiology, May 2000, p. 2208-2210, Vol. 66, No. 5
0099-2240/00/$04.00+0

Evaluation of a Fluorescent Lectin-Based Staining Technique for Some Acidophilic Mining Bacteria

Dee Jay Fife, Debby F. Bruhn, Karen S. Miller, and Daphne L. Stoner*

Biotechnology, Idaho National Engineering and Environmental Laboratory, Idaho Falls, Idaho 83415

Received 18 October 1999/Accepted 17 January 2000

A fluorescence-labeled wheat germ agglutinin staining technique (R. K. Sizemore et al., Appl. Environ. Microbiol. 56:2245-2247, 1990) was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.

* Corresponding author. Mailing address: Biotechnology, Idaho National Engineering and Environmental Laboratory, P.O. Box 1625, MS 2203, 2351 North Blvd., Idaho Falls, ID 83415. Phone: (208) 526-8786. Fax: (208) 526-0828. E-mail: dstoner{at}inel.gov.

Applied and Environmental Microbiology, May 2000, p. 2208-2210, Vol. 66, No. 5
0099-2240/00/$04.00+0


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