AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prioult, G.
Right arrow Articles by Fliss, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prioult, G.
Right arrow Articles by Fliss, I.
Agricola
Right arrow Articles by Prioult, G.
Right arrow Articles by Fliss, I.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2000, p. 2216-2219, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Simultaneous Immunofluorescent Detection of Coentrapped Cells in Gel Beads

Guenolee Prioult, Christophe Lacroix, Carl Turcotte, and Ismaïl Fliss*

Dairy Research Centre STELA, Pavillon Paul Comtois, Université Laval, Quebec, Canada G1K 7P4

Received 15 September 1999/Accepted 11 February 2000

An immunofluorescent method involving double color labeling and confocal microscopy was reported to specifically detect lactic acid bacteria and probiotic cells coimmobilized in gels beads. The method described is rapid (4 h) and sensitive and may be useful for studying cell dynamics during mixed-culture starter production using immobilized cells in gel beads. Microscopic observations were perfectly correlated to cell counts obtained using a sandwich enzyme-linked immunosorbent assay.

* Corresponding author. Mailing address: Dairy Research Centre STELA, Pavillon Paul Comtois, Université Laval, Quebec, Quebec Province, Canada. Phone: 418 656 2131, ext. 6825. Fax: 418 656 3353. E-mail: Ismail.Fliss{at}aln.ulaval.ca.

Applied and Environmental Microbiology, May 2000, p. 2216-2219, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2000 by the American Society for Microbiology. All rights reserved.