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Applied and Environmental Microbiology, May 2000, p. 2224-2226, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Design and Evaluation of a Lactobacillus manihotivorans Species-Specific rRNA-Targeted Hybridization Probe and Its Application to the Study of Sour Cassava Fermentation

Frédéric Ampe*

Institut de Recherche pour le Développement (IRD), Laboratoire de Biotechnologie Microbienne Tropicale, 34032 Montpellier, France

Received 15 November 1999/Accepted 23 February 2000

Based on 16S rRNA sequence comparison, we have designed a 20-mer oligonucleotide that targets a region specific to the species Lactobacillus manihotivorans recently isolated from sour cassava fermentation. The probe recognized the rRNA obtained from all the L. manihotivorans strains tested but did not recognize 56 strains of microorganisms from culture collections or directly isolated from sour cassava, including 29 species of lactic acid bacteria. This probe was then successfully used in quantitative RNA blots and demonstrated the importance of L. manihotivorans in the fermentation of sour cassava starch, which could represent up to 20% of total lactic acid bacteria.

* Mailing address: LBMRPM, CNRS-INRA BP 27, 31326 Castanet-Tolosan Cedex, France. Phone: 33 5 61 28 50 54. Fax: 33 5 61 28 50 61. E-mail: Frederic.Ampe{at}mpl.ird.fr.

Applied and Environmental Microbiology, May 2000, p. 2224-2226, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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