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Applied and Environmental Microbiology, May 2000, p. 2227-2231, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of a Single, Triplicate Arbitrarily Primed-PCR Procedure for Molecular Fingerprinting of Lactic Acid Bacteriadagger

Sean M. Cusick and Daniel J. O'Sullivan*

Department of Food Science and Nutrition and Department of Microbial Engineering, University of Minnesota, St. Paul, Minnesota 55108

Received 5 August 1999/Accepted 18 February 2000

Arbitrarily primed (AP)-PCR can be used to generate characteristic DNA fingerprint patterns. However, small changes in reaction conditions can cause band irreproducibility. In this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. A single triplicate AP-PCR (TAP-PCR) procedure, using an 18-mer primer, was developed and used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.

* Corresponding author. Mailing address: Department of Food Science and Nutrition, 1334 Eckles Ave., St. Paul, MN 55108. Phone: (612) 624-5335. Fax: (612) 625-5272. E-mail: dosulliv{at}tc.umn.edu.

dagger Published as paper number 001180009 of the Scientific Journal Series of the Minnesota Agricultural Experiment Station based on research conducted under project 18-055.

Applied and Environmental Microbiology, May 2000, p. 2227-2231, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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