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Applied and Environmental Microbiology, May 2000, p. 2235-2237, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Inactivation of the Glutamate Decarboxylase Gene in Lactococcus lactis subsp. cremoris

Masaru Nomura,* Miho Kobayashi, Sadahiro Ohmomo, and Takashi Okamoto

Department of Animal Products, National Institute of Animal Industry, Norindanchi, Tsukuba, Ibaraki 305-0901, Japan

Received 20 October 1999/Accepted 5 March 2000

Lactococcus lactis subsp. lactis strains show glutamate decarboxylase activity, whereas L. lactis subsp. cremoris strains do not. The gadB gene encoding glutamate decarboxylase was detected in the L. lactis subsp. cremoris genome but was poorly expressed. Sequence analysis showed that the gene is inactivated by the frameshift mutation and encoded in a nonfunctional protein.

* Corresponding author. Mailing address: Department of Animal Products, National Institute of Animal Industry, Norindanchi, Tsukuba, Ibaraki 305-0901, Japan. Phone and fax: 81-298-38-8688. E-mail: nomura{at}niai.affrc.go.jp.

Applied and Environmental Microbiology, May 2000, p. 2235-2237, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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