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Applied and Environmental Microbiology, May 2000, p. 2238-2242, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A New System for Three-Dimensional Tracking of Motile Microorganisms

Roland Thar,* Nicholas Blackburn, and Michael Kühl

Marine Biological Laboratory, University of Copenhagen, 3000 Helsingør, Denmark

Received 22 December 1999/Accepted 7 March 2000

A new three-dimensional (3D)-tracking system with optimized dark-field illumination is presented. It allows simultaneous 3D tracking of several free-swimming microorganisms with diameters of >10 µm. Resolution limits and illumination efficiencies for different size classes of microorganisms are treated analytically. First applications for 3D tracking of protists are demonstrated.

* Corresponding author. Mailing address: Marine Biological Laboratory, University of Copenhagen, Strandpromenaden 5, 3000 Helsingør, Denmark. Phone: 45 49 21 16 33 320/261. Fax: 45 49 26 11 65. E-mail: roland.thar{at}gmx.net.

Applied and Environmental Microbiology, May 2000, p. 2238-2242, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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