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Applied and Environmental Microbiology, May 2000, p. 2259-2262, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Fortified Sera and Their Use in Environmental Virology

Jonathan L. Hoyt and Aaron B. Margolin*

Department of Microbiology, Rudman Hall, University of New Hampshire, Durham, New Hampshire 03824

Received 8 July 1999/Accepted 15 February 2000

Four commercially available fortified sera were compared to fetal bovine serum (FBS) with regard to their ability to maintain or increase the sensitivity of the Buffalo green monkey (BGM) kidney cell line to viral infection. Nine virus strains and five wastewater samples were used. Fortified sera were comparable to FBS for the enumeration of some viruses by the plaque method and for the detection of virus in wastewater by the most-probable-number assay.

* Corresponding author. Mailing address: Department of Microbiology, Rudman Hall, Rm. 235, University of New Hampshire, Durham, NH 03824. Phone: (603) 862-2252. Fax: (603) 862-2621. E-mail: aaronm{at}christa.unh.edu.

Applied and Environmental Microbiology, May 2000, p. 2259-2262, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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