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Applied and Environmental Microbiology, May 2000, p. 2263-2266, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Quantification of Bacterial Groups within Human Fecal Flora by Oligonucleotide Probe Hybridization

Abdelghani Sghir,1,* Genevieve Gramet,1 Antonia Suau,1 Violaine Rochet,1 Philippe Pochart,2 and Joel Dore1

Institut National de Recherche Agronomique, Unité d'Ecologie et Physiologie du Système Digestif, Domaine de Vilvert, 78352 Jouy-en-Josas,1 and Conservatoire National des Arts et Métiers, 75141 Paris,2 France

Received 21 October 1999/Accepted 22 February 2000

To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed, validated, and used with a set of five 16S rRNA-targeted oligonucleotide probes. Application of the six probes to fecal samples from 27 human adults showed additivity of 70% of the total 16S rRNA detected by the bacterial domain probe. The Bacteroides group-specific probe accounted for 37% ± 16% of the total rRNA, while the enteric group probe accounted for less than 1%. Clostridium leptum subgroup and Clostridium coccoides group-specific probes accounted for 16% ± 7% and 14% ± 6%, respectively, while Bifidobacterium and Lactobacillus groups made up less than 2%.

* Corresponding author. Mailing address: Genoscope, 2, rue Gaston Crémieux, BP 5706, 91057 Evry Cedex, France. Phone: 01 60 87 25 31. Fax: 01 60 87 25 14. E-mail: sghir{at}genoscope.cns.fr.

Applied and Environmental Microbiology, May 2000, p. 2263-2266, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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