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Applied and Environmental Microbiology, May 2000, p. 2269-2273, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Activities of Garlic Oil, Garlic Powder, and Their Diallyl Constituents against Helicobacter pylori

E. A. O'Gara,1 D. J. Hill,2 and D. J. Maslin1,*

School of Health Sciences, University of Wolverhampton, Wolverhampton WV1 1DJ,1 and School of Applied Sciences, University of Wolverhampton, Wolverhampton WV1 1SB,2 United Kingdom

Received 18 October 1999/Accepted 9 February 2000

Chronic Helicobacter pylori disease is reduced with Allium vegetable intake. This study was designed to assess the in vivo anti-H. pylori potential of a variety of garlic substances. The garlic materials all showed substantial but widely differing anti-H. pylori effects against all strains and isolates tested. The MICs (range, 8 to 32 µg/ml) and minimum bactericidal concentrations (MBCs) (range, 16 to 32 µg/ml) of undiluted garlic oil (GO) were smaller than those of garlic powder (GP) (MIC range, 250 to 500 µg/ml; MBC range, 250 to 500 µg/ml) but greater than the MIC of allicin (4.0 µg/ml) (Table 2) present in GP. Allicin (MIC, 6 µg/ml; MBC, 6 µg/ml) was more potent than diallyl disulfide (MIC range, 100 to 200 µg/ml; MBC range, 100 to 200 µg/ml), its corresponding sulfide, but of a strength similar to that of diallyl tetrasulfide (MIC range, 3 to 6 µg/ml; MBC range, 3 to 6 µg/ml). Antimicrobial activity of the diallyl sulfides increased with the number of sulfur atoms. Time course viability studies and microscopy showed dose-dependent anti-H. pylori effects with undiluted GO, GP, allicin, and diallyl trisulfide after a lag phase of ca. 1 to 2 h. Substantial in vitro anti-H. pylori effects of pure GO and GP and their diallyl sulfur components exist, suggesting their potential for in vivo clinical use against H. pylori infections.

* Corresponding author. Mailing address: School of Health Sciences, University of Wolverhampton, 62-68 Lichfield St., Wolverhampton WV1 1DJ, United Kingdom. Phone: 01902 321138. Fax: 01902 321161. E-mail: bs2910{at}wlv.ac.uk.

Applied and Environmental Microbiology, May 2000, p. 2269-2273, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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