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Applied and Environmental Microbiology, May 2000, p. 2278-2281, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Production and Characterization of Monoclonal Antibodies against Vegetative Cells of Bacillus cereus

Nadine Charni, Claude Perissol, Jean Le Petit, and Nathalie Rugani*

Laboratoire de Microbiologie, Service 452, UPRES A 6116 CNRS, Faculté des Sciences et Techniques de St Jérôme, Université Aix-Marseille, 13397 Marseille Cedex 20, France

Received 20 December 1999/Accepted 3 March 2000

Two monoclonal antibodies (MAbs) against Bacillus cereus were produced. The MAbs (8D3 and 9B7) were selected by enzyme-linked immunosorbent assay for their reactivity with B. cereus vegetative cells. They reacted with B. cereus vegetative cells while failing to recognize B. cereus spores. Immunoblotting revealed that MAb 8D3 recognized a 22-kDa antigen, while MAb 9B7 recognized two antigens with molecular masses of approximately 58 and 62 kDa. The use of MAbs 8D3 and 9B7 in combination to develop an immunological method for the detection of B. cereus vegetative cells in foods was investigated.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Service 452, UPRES A 6116 CNRS, Faculté des Sciences et Techniques de St Jérôme, Université Aix-Marseille, Avenue Escadrille Normandie Niemen, 13397 Marseille Cedex 20, France. Phone: (33) 4 91 28 81 90. Fax: (33) 4 91 28 80 30. E-mail: Nathalie.Rugani{at}Microbio.u-3mrs.fr.

Applied and Environmental Microbiology, May 2000, p. 2278-2281, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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