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Applied and Environmental Microbiology, June 2000, p. 2349-2357, Vol. 66, No. 6
Department of Biological Sciences, University
of Stirling, Stirling FK9 4LA,1 and
School of Ocean and Earth Sciences, University of Southampton,
Southampton Oceanography Centre, Southampton SO14
3ZH,3 United Kingdom, and Department of
Earth and Ocean Sciences (Oceanography), University of British
Columbia, Vancouver, Canada V62 1242
Received 26 October 1999/Accepted 13 March 2000
Generic taxon-specific DNA probes that target an internal region of
the gene (rbcL) encoding the large subunit of
ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) were
developed for two groups of marine phytoplankton (diatoms and
prymnesiophytes). The specificity and utility of the probes were
evaluated in the laboratory and also during a 1-month mesocosm
experiment in which we investigated the temporal variability in RubisCO
gene expression and primary production in response to inorganic
nutrient enrichment. We found that the onset of successive bloom events
dominated by each of the two classes of chromophyte algae was
associated with marked taxon-specific increases in rbcL
transcription rates. These observations suggest that measurements of
RubisCO gene expression can provide an early indicator of the
development of phytoplankton blooms and may also be useful in
predicting which taxa are likely to dominate a bloom.
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Molecular and Physiological Responses of Two
Classes of Marine Chromophytic Phytoplankton (Diatoms and
Prymnesiophytes) during the Development of Nutrient-Stimulated
Blooms
and
*
Corresponding author. Mailing address: Department of
Biological Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom. Phone: (44) 1786 467784. Fax: (44) 1786 464994. E-mail: michael.wyman{at}stir.ac.uk.
Present address: School of Ocean and Earth Sciences, University of
Southampton, Southampton Oceanography Centre, Southampton SO14 3ZH,
United Kingdom.
Applied and Environmental Microbiology, June 2000, p. 2349-2357, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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