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Applied and Environmental Microbiology, June 2000, p. 2378-2384, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Macrocyclic Peptide Antibiotic Micrococcin P1 Is Secreted by the Food-Borne Bacterium Staphylococcus equorum WS 2733 and Inhibits Listeria monocytogenes on Soft Cheese

Markus C. Carnio,1 Alexandra Höltzel,2 Melanie Rudolf,1 Thomas Henle,3 Günther Jung,2 and Siegfried Scherer1,*

Institut für Mikrobiologie, FML Weihenstephan, Technische Universität München, D-85354 Freising,1 Institut für Organische Chemie, Universität Tübingen, D-72076 Tübingen,2 and Institut für Lebensmittelchemie, Technische Universität Dresden, D-01062 Dresden,3 Germany

Received 12 November 1999/Accepted 21 March 2000

Staphylococcus equorum WS 2733 was found to produce a substance exhibiting a bacteriostatic effect on a variety of gram-positive bacteria. The metabolite was purified to homogeneity by ammonium sulfate precipitation and semipreparative reversed-phase high-performance liquid chromatography. Electrospray mass spectrometry confirmed the high purity of the compound and revealed a molecular mass of 1,143 Da. By two-dimensional nuclear magnetic resonance spectroscopy the substance was identified as micrococcin P1 which is a macrocyclic peptide antibiotic that has not yet been reported for the genus Staphylococcus. A total of 95 out of 95 Listeria strains and 130 out of 135 other gram-positive bacteria were inhibited by this substance, while none of 37 gram-negative bacteria were affected. The antilisterial potential of this food-grade strain as a protective starter culture was evaluated by its in situ application in cheese-ripening experiments under laboratory conditions. A remarkable growth reduction of Listeria monocytogenes could be achieved compared to control cheese ripened with a nonbacteriocinogenic type strain of Staphylococcus equorum. In order to prove that inhibition was due to micrococcin P1, a micrococcin-deficient mutant was constructed which did not inhibit L. monocytogenes in cheese-ripening experiments.

* Corresponding author. Mailing address: Institut für Mikrobiologie, FML Weihen Stephan, Technische Universitat Munchen, D-85354 Freising, Germany. Phone: 49-8161-713516. Fax: 49-8161-714512. E-mail: Siegfried.Scherer{at}lrz.tum.de.

Applied and Environmental Microbiology, June 2000, p. 2378-2384, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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