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Applied and Environmental Microbiology, June 2000, p. 2392-2399, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Mycobacterium Strain with Extended Capacities for Degradation of Gasoline Hydrocarbons

Floriane Solano-Serena,1 Rémy Marchal,1,* Serge Casarégola,2 Christelle Vasnier,1 Jean-Michel Lebeault,3 and Jean-Paul Vandecasteele1

Département Microbiologie, Institut Français du Pétrole, 92852 Rueil-Malmaison,1 Laboratoire de Génétique Moléculaire et Cellulaire, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon,2 and Centre de Recherches de Royallieu, Université de Technologie de Compiègne, 60205 Compiègne Cedex,3 France

Received 15 November 1999/Accepted 21 March 2000

A bacterial strain (strain IFP 2173) was selected from a gasoline-polluted aquifer on the basis of its capacity to use 2,2,4-trimethylpentane (isooctane) as a sole carbon and energy source. This isolate, the first isolate with this capacity to be characterized, was identified by 16S ribosomal DNA analysis, and 100% sequence identity with a reference strain of Mycobacterium austroafricanum was found. Mycobacterium sp. strain IFP 2173 used an unusually wide spectrum of hydrocarbons as growth substrates, including n-alkanes and multimethyl-substituted isoalkanes with chains ranging from 5 to 16 carbon atoms long, as well as substituted monoaromatic hydrocarbons. It also attacked ethers, such as methyl t-butyl ether. During growth on gasoline, it degraded 86% of the substrate. Our results indicated that strain IFP 2173 was capable of degrading 3-methyl groups, possibly by a carboxylation and deacetylation mechanism. Evidence that it attacked the quaternary carbon atom structure by an as-yet-undefined mechanism during growth on 2,2,4-trimethylpentane and 2,2-dimethylpentane was also obtained.

* Corresponding author. Mailing address: Division Chimie et Physico-Chimie appliquées, Département de Microbiologie, Institut Français du Pétrole, 1 et 4 avenue de Bois Préau, 92852 Rueil-Malmaison, France. Phone: 33 1 47 52 69 24. Fax: 33 1 47 52 70 01. E-mail: remy.marchal{at}ifp.fr.

Applied and Environmental Microbiology, June 2000, p. 2392-2399, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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