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Applied and Environmental Microbiology, June 2000, p. 2471-2478, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Influence of Cadmium and Mercury on Activities of Ligninolytic Enzymes and Degradation of Polycyclic Aromatic Hydrocarbons by Pleurotus ostreatus in Soil

Petr Baldrian,1,* Carsten in der Wiesche,2 Jirí Gabriel,1 Frantisek Nerud,1 and Frantisek Zadrazil2

Institute of Microbiology, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic,1 and Institute of Plant Nutrition and Soil Science, FAL, 38116 Braunschweig, Germany2

Received 10 January 2000/Accepted 4 April 2000

The white-rot fungus Pleurotus ostreatus was able to degrade the polycyclic aromatic hydrocarbons (PAHs) benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]anthracene, and benzo[ghi]perylene in nonsterile soil both in the presence and in the absence of cadmium and mercury. During 15 weeks of incubation, recovery of individual compounds was 16 to 69% in soil without additional metal. While soil microflora contributed mostly to degradation of pyrene (82%) and benzo[a]anthracene (41%), the fungus enhanced the disappearance of less-soluble polycyclic aromatic compounds containing five or six aromatic rings. Although the heavy metals in the soil affected the activity of ligninolytic enzymes produced by the fungus (laccase and Mn-dependent peroxidase), no decrease in PAH degradation was found in soil containing Cd or Hg at 10 to 100 ppm. In the presence of cadmium at 500 ppm in soil, degradation of PAHs by soil microflora was not affected whereas the contribution of fungus was negligible, probably due to the absence of Mn-dependent peroxidase activity. In the presence of Hg at 50 to 100 ppm or Cd at 100 to 500 ppm, the extent of soil colonization by the fungus was limited.

* Corresponding author. Mailing address: Institute of Microbiology, ASCR, Vídenská 1083, 14220 Prague 4, Czech Republic. Phone: 420 2 475 2315. Fax: 420 2 475 2396. E-mail: baldrian{at}biomed.cas.cz.

Applied and Environmental Microbiology, June 2000, p. 2471-2478, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Valaskova, V., Baldrian, P. (2006). Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus - production of extracellular enzymes and characterization of the major cellulases. Microbiology 152: 3613-3622 [Abstract] [Full Text]  
  • Szolar, O. H. J., Rost, H., Hirmann, D., Hasinger, M., Braun, R., Loibner, A. P. (2004). Sequential Supercritical Fluid Extraction (SSFE) for Estimating the Availability of High Molecular Weight Polycyclic Aromatic Hydrocarbons in Historically Polluted Soils. J. Environ. Qual. 33: 80-88 [Abstract] [Full Text]  
  • Steffen, K. T., Hatakka, A., Hofrichter, M. (2003). Degradation of Benzo[a]pyrene by the Litter-Decomposing Basidiomycete Stropharia coronilla: Role of Manganese Peroxidase. Appl. Environ. Microbiol. 69: 3957-3964 [Abstract] [Full Text]  
  • Alcalde, M., Bulter, T., Arnold, F. H. (2002). Colorimetric Assays for Biodegradation of Polycyclic Aromatic Hydrocarbons by Fungal Laccases. J Biomol Screen 7: 547-553 [Abstract]  
  • Baldrian, P., Gabriel, J. (2002). Intraspecific variability in growth response to cadmium of the wood-rotting fungus Piptoporus betulinus. Mycologia 94: 428-436 [Abstract] [Full Text]  


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