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Applied and Environmental Microbiology, June 2000, p. 2565-2571, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Biosynthesis of the Lantibiotic Mersacidin:
Organization of a Type B Lantibiotic Gene Cluster
Karsten
Altena,
André
Guder,
Claudia
Cramer, and
Gabriele
Bierbaum*
Institut für Medizinische Mikrobiologie
und Immunologie der Universität Bonn, D-53105 Bonn, Germany
Received 29 November 1999/Accepted 4 April 2000
The biosynthetic gene cluster (12.3 kb) of mersacidin, a
lanthionine-containing antimicrobial peptide, is located on the
chromosome of the producer, Bacillus sp. strain HIL
Y-85,54728 in a region that corresponds to 348° on the chromosome of
Bacillus subtilis 168. It consists of 10 open reading
frames and contains, in addition to the previously described mersacidin
structural gene mrsA (G. Bierbaum, H. Brötz, K.-P.
Koller, and H.-G. Sahl, FEMS Microbiol. Lett. 127:121-126, 1995), two
genes, mrsM and mrsD, coding for enzymes
involved in posttranslational modification of the prepeptide; one gene,
mrsT, coding for a transporter with an associated protease domain; and three genes, mrsF, mrsG, and
mrsE, encoding a group B ABC transporter that could be
involved in producer self-protection. Additionally, three regulatory
genes are part of the gene cluster, i.e., mrsR2 and
mrsK2, which encode a two-component regulatory system which
seems to be necessary for the transcription of the mrsFGE
operon, and mrsR1, which encodes a protein with similarity to response regulators. Transcription of mrsA sets in at
early stationary phase (between 8 and 16 h of culture).
*
Corresponding author. Mailing address: Institut
für Medizinische Mikrobiologie und Immunologie der
Universität Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany.
Phone: 49-228-287-9103. Fax: 49-228-287-4808. E-mail:
bierbaum{at}mibi03.meb.uni-bonn.de.

Present address: Abt. Biotechnik, Pharma-Zentrale GmbH, D-58313
Herdecke,
Germany.
Applied and Environmental Microbiology, June 2000, p. 2565-2571, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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