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Applied and Environmental Microbiology, June 2000, p. 2631-2635, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning, Expression, and Sequence Analysis of the Gene Encoding the Alkali-Stable, Thermostable Endoxylanase from Alkalophilic, Mesophilic Bacillus sp. Strain NG-27

Naveen Gupta,1 Vanga Shiva Reddy,2 Sankar Maiti,1 and Amit Ghosh1,*

Institute of Microbial Technology, Sector 39-A, Chandigarh-160036,1 and International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067,2 India

Received 11 October 1999/Accepted 10 March 2000

Alkalophilic Bacillus sp. strain NG-27 produces a 42-kDa endoxylanase active at 70°C and at a pH of 8.4. The gene for this endoxylanase was cloned and sequenced. The gene contained one open reading frame of 1,215 bases. An active site characteristic of the family 10 beta -glycanases was recognized between amino acids 303 and 313, with the active glutamate at position 310. Though highly thermostable, the enzyme contains no cysteine residue.

* Corresponding author. Mailing address: Institute of Microbial Technology, Sector 39-A, Chandigarh-160036, India. Phone: 91-172-690785/690684. Fax: 91-172-690585/690632. E-mail: director{at}lion.imtech.ernet.in.

Applied and Environmental Microbiology, June 2000, p. 2631-2635, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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