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Applied and Environmental Microbiology, June 2000, p. 2668-2672, Vol. 66, No. 6
0099-2240/00/$04.00+0

trans-o-Hydroxybenzylidenepyruvate Hydratase-Aldolase as a Biocatalystdagger

Richard W. Eaton*

Gulf Ecology Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Gulf Breeze, Florida 32561

Received 7 February 2000/Accepted 31 March 2000

The hydratase-aldolase-catalyzed conversion of trans-o-hydroxybenzylidenepyruvate to salicylaldehyde and pyruvate is an intermediate reaction in the conversion of naphthalene to salicylate by bacteria. Here, a variety of aromatic aldehydes and some nonaromatic aldehydes together with pyruvate have been shown to be substrates for aldol condensations catalyzed by this enzyme in extracts of the recombinant strain Escherichia coli JM109(pRE701). Some of the products of these reactions were also compared as substrates in the opposite (hydration-aldol cleavage) reaction.

* Mailing address: Gulf Ecology Division, NHEERL, U.S. Environmental Protection Agency, 1 Sabine Island Dr., Gulf Breeze, FL 32561. Phone: (850) 934-9345. Fax: (850) 934-9201. E-mail: eaton.richard{at}epa.gov.

dagger Contribution 1095 from the Gulf Ecology Division, NHEERL, U.S. Environmental Protection Agency, Gulf Breeze, Fla.

Applied and Environmental Microbiology, June 2000, p. 2668-2672, Vol. 66, No. 6
0099-2240/00/$04.00+0




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