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Applied and Environmental Microbiology, June 2000, p. 2685-2689, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation of a Pandemic O3:K6 Clone of a Vibrio parahaemolyticus Strain from Environmental and Clinical Sources in Thailand

Varaporn Vuddhakul,1,2 Ashrafazzuman Chowdhury,2 Varaporn Laohaprertthisan,3 Punnee Pungrasamee,1 Nuanjira Patararungrong,1 Pongpaga Thianmontri,1 Masanori Ishibashi,4 Chiho Matsumoto,2 and Mitsuaki Nishibuchi2,*

Department of Microbiology, Faculty of Science,1 and Department of Pathology, Faculty of Medicine,3 Prince of Songkla University, Hat-yai, Thailand, and Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto,2 and Osaka Prefectural Institute of Public Health, Higashinari-ku, Osaka,4 Japan

Received 14 January 2000/Accepted 27 March 2000

Application of an immunomagnetic enrichment method selective for Vibrio parahaemolyticus serovar K6 allowed isolation of a strain belonging to the pandemic O3:K6 clone of V. parahaemolyticus from fresh shellfish not implicated in a clinical case in southern Thailand. Arbitrarily primed PCR profiles of this strain, clinical O3:K6 strains isolated from sporadic diarrhea cases in the same area, and a standard pandemic O3:K6 strain were indistinguishable.

* Corresponding author. Mailing address: Center for Southeast Asian Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. Phone: 81-75-753-7367. Fax: 81-75-753-7350. E-mail: nishibuc{at}mb.med.kyoto-u.ac.jp.

Applied and Environmental Microbiology, June 2000, p. 2685-2689, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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