This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ward, B. B.
Right arrow Articles by Joye, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ward, B. B.
Right arrow Articles by Joye, S. B.
Agricola
Right arrow Articles by Ward, B. B.
Right arrow Articles by Joye, S. B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2000, p. 2873-2881, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Analysis of Ammonia-Oxidizing Bacteria from Hypersaline Mono Lake, California, on the Basis of 16S rRNA Sequences

B. B. Ward,1,dagger ,* D. P. Martino,2,dagger M. C. Diaz,3 and S. B. Joye2,Dagger

Institute of Marine Sciences1 and Biology Department,3 University of California, Santa Cruz, California, and Department of Oceanography, Texas A & M University, College Station, Texas 778432

Received 11 October 1999/Accepted 2 May 2000

Ammonia-oxidizing bacteria were detected by PCR amplification of DNA extracted from filtered water samples throughout the water column of Mono Lake, California. Ammonia-oxidizing members of the beta  subdivision of the division Proteobacteria (beta -subdivision Proteobacteria) were detected using previously characterized PCR primers; target sequences were detected by direct amplification in both surface water and below the chemocline. Denaturing gradient gel electrophoresis analysis indicated the presence of at least four different beta -subdivision ammonia oxidizers in some samples. Subsequent sequencing of amplified 16S rDNA fragments verified the presence of sequences very similar to those of cultured Nitrosomonas strains. Two separate analyses, carried out under different conditions (different reagents, locations, PCR machines, sequencers, etc.), 2 years apart, detected similar ranges of sequence diversity in these samples. It seems likely that the physiological diversity of nitrifiers exceeds the diversity of their ribosomal sequences and that these sequences represent members of the Nitrosomonas europaea group that are acclimated to alkaline, high-salinity environments. Primers specific for Nitrosococcus oceanus, a marine ammonia-oxidizing bacterium in the gamma  subdivision of the Proteobacteria, did not amplify target from any samples.

* Corresponding author. Mailing address: Geosciences Department, Princeton University, Princeton, NJ 08544. Phone: (609) 258-5150. Fax: (609) 258-1274. E-mail: bbw{at}princeton.edu.

dagger Present address: Geosciences Department, Princeton University, Princeton, NJ 08544.

Dagger Present address: Department of Marine Sciences, University of Georgia, Athens, GA 30602.

Applied and Environmental Microbiology, July 2000, p. 2873-2881, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Junier, P., Kim, O.-S., Hadas, O., Imhoff, J. F., Witzel, K.-P. (2008). Evaluation of PCR Primer Selectivity and Phylogenetic Specificity by Using Amplification of 16S rRNA Genes from Betaproteobacterial Ammonia-Oxidizing Bacteria in Environmental Samples. Appl. Environ. Microbiol. 74: 5231-5236 [Abstract] [Full Text]  
  • Coci, M., Bodelier, P. L. E., Laanbroek, H. J. (2008). Epiphyton as a Niche for Ammonia-Oxidizing Bacteria: Detailed Comparison with Benthic and Pelagic Compartments in Shallow Freshwater Lakes. Appl. Environ. Microbiol. 74: 1963-1971 [Abstract] [Full Text]  
  • Molina, V., Ulloa, O., Farias, L., Urrutia, H., Ramirez, S., Junier, P., Witzel, K.-P. (2007). Ammonia-Oxidizing {beta}-Proteobacteria from the Oxygen Minimum Zone off Northern Chile. Appl. Environ. Microbiol. 73: 3547-3555 [Abstract] [Full Text]  
  • Lin, J.-L., Joye, S. B., Scholten, J. C. M., Schafer, H., McDonald, I. R., Murrell, J. C. (2005). Analysis of Methane Monooxygenase Genes in Mono Lake Suggests That Increased Methane Oxidation Activity May Correlate with a Change in Methanotroph Community Structure. Appl. Environ. Microbiol. 71: 6458-6462 [Abstract] [Full Text]  
  • Freitag, T. E., Prosser, J. I. (2003). Community Structure of Ammonia-Oxidizing Bacteria within Anoxic Marine Sediments. Appl. Environ. Microbiol. 69: 1359-1371 [Abstract] [Full Text]  
  • Humayoun, S. B., Bano, N., Hollibaugh, J. T. (2003). Depth Distribution of Microbial Diversity in Mono Lake, a Meromictic Soda Lake in California. Appl. Environ. Microbiol. 69: 1030-1042 [Abstract] [Full Text]  
  • Oremland, R. S., Hoeft, S. E., Santini, J. M., Bano, N., Hollibaugh, R. A., Hollibaugh, J. T. (2002). Anaerobic Oxidation of Arsenite in Mono Lake Water and by a Facultative, Arsenite-Oxidizing Chemoautotroph, Strain MLHE-1. Appl. Environ. Microbiol. 68: 4795-4802 [Abstract] [Full Text]  
  • Ward, B. B., O'Mullan, G. D. (2002). Worldwide Distribution of Nitrosococcus oceani, a Marine Ammonia-Oxidizing {gamma}-Proteobacterium, Detected by PCR and Sequencing of 16S rRNA and amoA Genes. Appl. Environ. Microbiol. 68: 4153-4157 [Abstract] [Full Text]  
  • Zehr, J. P., Ward, B. B. (2002). Nitrogen Cycling in the Ocean: New Perspectives on Processes and Paradigms. Appl. Environ. Microbiol. 68: 1015-1024 [Full Text]  
  • Hollibaugh, J. T., Bano, N., Ducklow, H. W. (2002). Widespread Distribution in Polar Oceans of a 16S rRNA Gene Sequence with Affinity to Nitrosospira-Like Ammonia-Oxidizing Bacteria. Appl. Environ. Microbiol. 68: 1478-1484 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»