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Applied and Environmental Microbiology, July 2000, p. 3058-3064, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Introduction of Raw Starch-Binding Domains into Bacillus subtilis alpha -Amylase by Fusion with the Starch-Binding Domain of Bacillus Cyclomaltodextrin Glucanotransferase

Kohji Ohdan,1 Takashi Kuriki,1,* Hiroki Takata,1 Hiroki Kaneko,2 and Shigetaka Okada1

Biochemical Research Laboratory, Ezaki Glico Co., Ltd., Utajima 4-6-5, Nishiyodogawa-ku, Osaka 555-8502,1 and Fundamental Research Laboratories, NEC Corporation, Miyukigaoka, Tsukuba, Ibaraki 305-0841,2 Japan

Received 25 February 2000/Accepted 18 April 2000

We constructed two types of chimeric enzymes, Ch1 Amy and Ch2 Amy. Ch1 Amy consisted of a catalytic domain of Bacillus subtilis X-23 alpha -amylase (Ba-S) and the raw starch-binding domain (domain E) of Bacillus A2-5a cyclomaltodextrin glucanotransferase (A2-5a CGT). Ch2 Amy consisted of Ba-S and D (function unknown) plus E domains of A2-5a CGT. Ch1 Amy acquired raw starch-binding and -digesting abilities which were not present in the catalytic part (Ba-S). Furthermore, the specific activity of Ch1 Amy was almost identical when enzyme activity was evaluated on a molar basis. Although Ch2 Amy exhibited even higher raw starch-binding and -digesting abilities than Ch1 Amy, the specific activity was lower than that of Ba-S. We did not detect any differences in other enzymatic characteristics (amylolytic pattern, transglycosylation ability, effects of pH, and temperature on stability and activity) among Ba-S, Ch1 Amy, and Ch2 Amy.

* Corresponding author. Mailing address: Biochemical Research Laboratory, Ezaki Glico Co., Ltd., Utajima 4-6-5, Nishiyodogawa-ku, Osaka 555-8502, Japan. Phone: 81-6-6477-8425. Fax: 81-6-6477-8362. E-mail: kuriki-takashi{at}glico.co.jp.

Applied and Environmental Microbiology, July 2000, p. 3058-3064, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cornett, C. A.G., Fang, T.-Y., Reilly, P. J., Ford, C. (2003). Starch-binding domain shuffling in Aspergillus niger glucoamylase. Protein Eng Des Sel 16: 521-529 [Abstract] [Full Text]  
  • Kamasaka, H., Sugimoto, K., Takata, H., Nishimura, T., Kuriki, T. (2002). Bacillus stearothermophilus Neopullulanase Selective Hydrolysis of Amylose to Maltose in the Presence of Amylopectin. Appl. Environ. Microbiol. 68: 1658-1664 [Abstract] [Full Text]  


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