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Applied and Environmental Microbiology, July 2000, p. 3088-3092, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Naphthalene and Donor Cell Density Influence Field Conjugation of Naphthalene Catabolism Plasmids

A. M. Hohnstock, K. G. Stuart-Keil, E. E. Kull, and E. L. Madsen*

Section of Microbiology, Division of Biological Sciences, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York 14853-8101

Received 28 December 1999/Accepted 12 April 2000

We examined transfer of naphthalene-catabolic genes from donor microorganisms native to a contaminated site to site-derived, rifampin-resistant recipient bacteria unable to grow on naphthalene. Horizontal gene transfer (HGT) was demonstrated in filter matings using groundwater microorganisms as donors. Two distinct but similar plasmid types, closely related to pDTG1, were retrieved. In laboratory-incubated sediment matings, the addition of naphthalene stimulated HGT. However, recipient bacteria deployed in recoverable vessels in the field site (in situ) did not retrieve plasmids from native donors. Only when plasmid-containing donor cells and naphthalene were added to the in situ mating experiments did HGT occur.

* Corresponding author. Mailing address: Section of Microbiology, Division of Biological Sciences, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853-8101. Phone: (607) 255-3086. Fax: (607) 255-3904. E-mail: elm3{at}cornell.edu.

Applied and Environmental Microbiology, July 2000, p. 3088-3092, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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