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Applied and Environmental Microbiology, July 2000, p. 3102-3109, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of 16S Ribosomal DNA-Defined Bacterial Populations at a Shallow Submarine Hydrothermal Vent near Milos Island (Greece)

Stefan M. Sievert,1,* Jan Kuever,1,* and Gerard Muyzer2,dagger

Department of Microbiology1 and Molecular Ecology Group,2 Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany

Received 3 December 1999/Accepted 17 April 2000

In a recent publication (S. M. Sievert, T. Brinkhoff, G. Muyzer, W. Ziebis, and J. Kuever, Appl. Environ. Microbiol. 65:3834-3842, 1999) we described spatiotemporal changes in the bacterial community structure at a shallow-water hydrothermal vent in the Aegean Sea near the isle of Milos (Greece). Here we describe identification and phylogenetic analysis of the predominant bacterial populations at the vent site and their distribution at the vent site as determined by sequencing of DNA molecules (bands) excised from denaturing gradient gels. A total of 36 bands could be sequenced, and there were representatives of eight major lineages of the domain Bacteria. Cytophaga-Flavobacterium and Acidobacterium were the most frequently retrieved bacterial groups. Less than 33% of the sequences exhibited 90% or more identity with cultivated organisms. The predominance of putative heterotrophic populations in the sequences retrieved is explained by the input of allochthonous organic matter at the vent site.

* Corresponding authors. Mailing address: Max Planck Institute for Marine Microbiology, Celsiusstr. 1, D-28359 Bremen, Germany. Phone: 49-421-2028-738 (S. M. Sievert) and 49-421-2028-734 (J. Kuever). Fax: 49-421-2028580. E-mail: ssievert{at}mpi-bremen.de (S. M. Sievert) and jkuever{at}mpi-bremen.de (J. Kuever).

dagger Present address: Netherlands Institute for Sea Research, NL-1790AB Den Burg (Texel), The Netherlands.

Applied and Environmental Microbiology, July 2000, p. 3102-3109, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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