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Applied and Environmental Microbiology, July 2000, p. 3113-3116, Vol. 66, No. 7
Abteilung Allgemeine
Mikrobiologie1 and Göttingen
Genomics Laboratory,2 Institut für
Mikrobiologie und Genetik der Georg-August-Universität, 37077 Göttingen, Germany
Received 24 January 2000/Accepted 5 April 2000
Environmental DNA libraries prepared from three different soil
samples were screened for genes conferring lipolytic activity on
Escherichia coli clones. Screening on triolein agar
revealed 1 positive clone out of 730,000 clones, and screening on
tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one
recombinant strain harbored a lipase and the other three contained
esterases. The genes responsible for the lipolytic activity were
identified and characterized.
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Screening of Environmental DNA Libraries for the
Presence of Genes Conferring Lipolytic Activity on
Escherichia coli
*
Corresponding author. Mailing address: Institut
für Mikrobiologie und Genetik der Georg-August-Universität,
Grisebachstr. 8, 37077 Göttingen, Germany. Phone: 49-551-393827. Fax: 49-551-393808. E-mail: rdaniel{at}gwdg.de.
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