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Applied and Environmental Microbiology, July 2000, p. 3117-3118, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Modification of Sorbitol MacConkey Medium Containing Cefixime and Tellurite for Isolation of Escherichia coli O157:H7 from Radish Sprouts

Tomohiko Fujisawa,1,* Shin Sata,1 Katsuhiro Aikawa,1 Takanori Takahashi,1 Shiro Yamai,1 and Toshio Shimada2

Kanagawa Prefectural Public Health Laboratory, Yokohama 241-0815,1 and National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640,2 Japan

Received 22 December 1999/Accepted 13 April 2000

A modified version of sorbitol MacConkey medium containing cefixime and tellurite (CT-SMAC medium) was produced by adding salicin and 4-methylumbelliferyl-beta -D-galactopyranoside to CT-SMAC medium; this medium was designated CT-SSMAC medium and was used to isolate Escherichia coli O157:H7 from radish sprouts. Of 101 non-E. coli bacteria isolated from radish sprouts that produced colorless colonies similar to colonies of E. coli O157:H7 grown on CT-SMAC medium, 92 (91%) formed colonies that were red to pink or were beta -galactosidase negative and colorless on CT-SSMAC medium. On the other hand, colonies of E. coli O157:H7 strains were colorless and beta -galactosidase positive on CT-SSMAC medium. Our results suggest that CT-SSMAC medium is more selective than CT-SMAC medium for isolating E. coli O157:H7.

* Corresponding author. Mailing address: Kanagawa Prefectural Public Health Laboratory, 1-1-1, Nakao, Asahi-ku, Yokohama, 241-0815, Japan. Phone: 045-363-1030. Fax: 045-363-1037.

Applied and Environmental Microbiology, July 2000, p. 3117-3118, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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