Applied and Environmental Microbiology, July 2000, p. 3117-3118, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Kanagawa Prefectural Public Health Laboratory, Yokohama 241-0815,1 and National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640,2 Japan
Received 22 December 1999/Accepted 13 April 2000
A modified version of sorbitol MacConkey medium containing cefixime
and tellurite (CT-SMAC medium) was produced by adding salicin and
4-methylumbelliferyl-
-D-galactopyranoside to CT-SMAC medium; this medium was designated CT-SSMAC medium and was used to
isolate Escherichia coli O157:H7 from radish sprouts. Of
101 non-E. coli bacteria isolated from radish sprouts that
produced colorless colonies similar to colonies of E. coli
O157:H7 grown on CT-SMAC medium, 92 (91%) formed colonies that were
red to pink or were
-galactosidase negative and colorless on
CT-SSMAC medium. On the other hand, colonies of E. coli
O157:H7 strains were colorless and
-galactosidase positive on
CT-SSMAC medium. Our results suggest that CT-SSMAC medium is more
selective than CT-SMAC medium for isolating E. coli
O157:H7.
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