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Applied and Environmental Microbiology, August 2000, p. 3241-3248, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Three-Year Study To Assess Human Enteric Viruses in Shellfish

F. Le Guyader,* L. Haugarreau, L. Miossec, E. Dubois,dagger and M. Pommepuy

Microbiology Laboratory, IFREMER, 44 311 Nantes Cedex 03, France

Received 1 November 1999/Accepted 17 May 2000

The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons showed a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or Norwalk virus). Viral contamination was mainly observed during winter months, although there were some seasonal differences among the viruses. This first study of virus detection over a fairly long period of time suggests that routine analysis of shellfish by a molecular technique is feasible.

* Corresponding author. Mailing address: Microbiology Laboratory, IFREMER, BP 21105, 44 311 Nantes Cedex 03, France. Phone: 33 2 40 37 40 52. Fax: 33 2 40 37 40 73. E-mail: sleguyad{at}ifremer.fr.

dagger Present address: Virology Laboratory, AFSSA, 94703 Maison Alfort Cedex, France.

Applied and Environmental Microbiology, August 2000, p. 3241-3248, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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