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Applied and Environmental Microbiology, August 2000, p. 3241-3248, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Three-Year Study To Assess Human Enteric Viruses in
Shellfish
F.
Le Guyader,*
L.
Haugarreau,
L.
Miossec,
E.
Dubois,
and
M.
Pommepuy
Microbiology Laboratory, IFREMER, 44 311 Nantes Cedex 03, France
Received 1 November 1999/Accepted 17 May 2000
The main pathogenic enteric viruses able to persist in the
environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV),
enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by
reverse transcription-PCR and hybridization in shellfish during a
3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage,
were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV
(45%), and RV (52%). Sequences obtained from HAV and NLV amplicons
showed a great variety of strains, especially for NLV (strains close to
Mexico, Snow Mountain Agent, or Norwalk virus). Viral contamination was
mainly observed during winter months, although there were some seasonal
differences among the viruses. This first study of virus detection over
a fairly long period of time suggests that routine analysis of
shellfish by a molecular technique is feasible.
*
Corresponding author. Mailing address: Microbiology
Laboratory, IFREMER, BP 21105, 44 311 Nantes Cedex 03, France. Phone: 33 2 40 37 40 52. Fax: 33 2 40 37 40 73. E-mail:
sleguyad{at}ifremer.fr.

Present address: Virology Laboratory, AFSSA, 94703 Maison Alfort
Cedex,
France.
Applied and Environmental Microbiology, August 2000, p. 3241-3248, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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