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Applied and Environmental Microbiology, August 2000, p. 3305-3309, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Polyamine Composition and Expression of Genes Related to Polyamine Biosynthesis in an Aphid Endosymbiont, Buchnera

Atsushi Nakabachidagger and Hajime Ishikawa*

Department of Biological Sciences, Graduate School of Sciences University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Received 10 March 2000/Accepted 26 May 2000

Polyamine composition in an aphid endosymbiotic bacterium, Buchnera sp., was determined by high-performance liquid chromatographic analysis. We found that Buchnera contained virtually only a single polyamine, spermidine. The spermidine content of Buchnera was considerably higher in young aphids and tended to decrease with the age of the host. Expression of speD and speE, whose gene products are key enzymes in the synthesis of spermidine, was analyzed by real-time quantitative reverse transcription-PCR. It was shown that the levels of their mRNAs fluctuated in line with the spermidine content.

* Corresponding author. Mailing address: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Phone: 81-3-5800-3553. Fax: 81-3-5800-3553. E-mail: iskw{at}biol.s.u-tokyo.ac.jp.

dagger Present address: Laboratory of Microbiology, RIKEN (The Institute of Physical and Chemical Research), Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan.

Applied and Environmental Microbiology, August 2000, p. 3305-3309, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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