This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feng, X.
Right arrow Articles by Widmer, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feng, X.
Right arrow Articles by Widmer, G.
Agricola
Right arrow Articles by Feng, X.
Right arrow Articles by Widmer, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2000, p. 3344-3349, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Extensive Polymorphism in Cryptosporidium parvum Identified by Multilocus Microsatellite Analysis

Xiaochuan Feng,1 Stephen M. Rich,1 Donna Akiyoshi,1 James K. Tumwine,2 Addy Kekitiinwa,2 Nicolette Nabukeera,2 Saul Tzipori,1 and Giovanni Widmer1,*

Tufts University School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, Massachusetts,1 and Mulago Hospital, Makerere University, Kampala, Uganda2

Received 27 March 2000/Accepted 17 May 2000

Restriction fragment length polymorphism and DNA sequence analysis discern two main types of Cryptosporidium parvum. We present a survey of length polymorphism at several microsatellite loci for type 1 and type 2 isolates. A total of 14 microsatellite loci were identified from C. parvum DNA sequences deposited in public databases. All repeats were mono-, di-, and trinucleotide repeats of A, AT, and AAT, reflecting the high AT content of the C. parvum genome. Several of these loci showed significant length polymorphism, with as many as seven alleles identified for a single locus. Differences between alleles ranged from 1 to 27 bp. Karyotype analysis using probes flanking three microsatellites localized each marker to an individual chromosomal band, suggesting that these markers are single copy. In a sample of 19 isolates for which at least three microsatellites were typed, a majority of isolates displayed a unique multilocus fingerprint. Microsatellite analysis of isolates passaged between different host species identified genotypic changes consistent with changes in parasite populations.

* Corresponding author. Mailing address: Tufts University School of Veterinary Medicine, Bldg. 20, 200 Westboro Rd., North Grafton, MA 01536. Phone: (508) 839-7944. Fax: (508) 839-7911. E-mail: gwidmer{at}infonet.tufts.edu.

Applied and Environmental Microbiology, August 2000, p. 3344-3349, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Leoni, F., Mallon, M. E., Smith, H. V., Tait, A., McLauchlin, J. (2007). Multilocus Analysis of Cryptosporidium hominis and Cryptosporidium parvum Isolates from Sporadic and Outbreak-Related Human Cases and C. parvum Isolates from Sporadic Livestock Cases in the United Kingdom. J. Clin. Microbiol. 45: 3286-3294 [Abstract] [Full Text]  
  • Tanriverdi, S., Markovics, A., Arslan, M. O., Itik, A., Shkap, V., Widmer, G. (2006). Emergence of Distinct Genotypes of Cryptosporidium parvum in Structured Host Populations. Appl. Environ. Microbiol. 72: 2507-2513 [Abstract] [Full Text]  
  • Xiao, L., Fayer, R., Ryan, U., Upton, S. J. (2004). Cryptosporidium Taxonomy: Recent Advances and Implications for Public Health. Clin. Microbiol. Rev. 17: 72-97 [Abstract] [Full Text]  
  • TUMWINE, J. K., KEKITIINWA, A., NABUKEERA, N., AKIYOSHI, D. E., RICH, S. M., WIDMER, G., FENG, X., TZIPORI, S. (2003). CRYPTOSPORIDIUM PARVUM IN CHILDREN WITH DIARRHEA IN MULAGO HOSPITAL, KAMPALA, UGANDA. Am J Trop Med Hyg 68: 710-715 [Abstract] [Full Text]  
  • Gasser, R. B., EL-Osta, Y. G. A., Chalmers, R. M. (2003). Electrophoretic Analysis of Genetic Variability within Cryptosporidium parvum from Imported and Autochthonous Cases of Human Cryptosporidiosis in the United Kingdom. Appl. Environ. Microbiol. 69: 2719-2730 [Abstract] [Full Text]  
  • Leoni, F., Gallimore, C. I., Green, J., McLauchlin, J. (2003). Molecular Epidemiological Analysis of Cryptosporidium Isolates from Humans and Animals by Using a Heteroduplex Mobility Assay and Nucleic Acid Sequencing Based on a Small Double-Stranded RNA Element. J. Clin. Microbiol. 41: 981-992 [Abstract] [Full Text]  
  • Akiyoshi, D. E., Feng, X., Buckholt, M. A., Widmer, G., Tzipori, S. (2002). Genetic Analysis of a Cryptosporidium parvum Human Genotype 1 Isolate Passaged through Different Host Species. Infect. Immun. 70: 5670-5675 [Abstract] [Full Text]  
  • Leav, B. A., Mackay, M. R., Anyanwu, A., O' Connor, R. M., Cevallos, A. M., Kindra, G., Rollins, N. C., Bennish, M. L., Nelson, R. G., Ward, H. D. (2002). Analysis of Sequence Diversity at the Highly Polymorphic Cpgp40/15 Locus among Cryptosporidium Isolates from Human Immunodeficiency Virus-Infected Children in South Africa. Infect. Immun. 70: 3881-3890 [Abstract] [Full Text]  
  • Straub, T. M., Daly, D. S., Wunshel, S., Rochelle, P. A., DeLeon, R., Chandler, D. P. (2002). Genotyping Cryptosporidium parvum with an hsp70 Single-Nucleotide Polymorphism Microarray. Appl. Environ. Microbiol. 68: 1817-1826 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»