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Applied and Environmental Microbiology, August 2000, p. 3415-3420, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Quantitative Determination of the Biodegradable Polymer Poly(beta -hydroxybutyrate) in a Recombinant Escherichia coli Strain by Use of Mid-Infrared Spectroscopy and Multivariative Statistics

Mustafa Kansiz,1 Helen Billman-Jacobe,2 and Don McNaughton1,*

Department of Chemistry1 and Department of Microbiology,2 Monash University, Melbourne, Victoria 3168, Australia

Received 8 February 2000/Accepted 9 May 2000

Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(beta -hydroxybutyrate) (PHB) contents of bacterial cells. A total of 237 replicate spectra from 34 samples were obtained together with gas chromatography-determined reference PHB contents. Using the PLS regression, we were able to relate the infrared spectra to the reference PHB contents, and the correlation coefficient between the measured and predicted values for the optimal model with a standard error of prediction of 1.49% PHB was 0.988. With this technique, there are no solvent requirements, sample preparation is minimal and simple, and analysis time is greatly reduced; our results demonstrate the potential of FTIR spectroscopy as an alternative to the conventional methods used for analysis of PHB in bacterial cells.

* Corresponding author. Mailing address: Department of Chemistry, Monash University, Wellington Rd., Clayton 3168, Victoria, Australia. Phone: 61 3 9905 4525. Fax: 61 3 9905 4597. E-mail: d.mcnaughton{at}sci.monash.edu.au.

Applied and Environmental Microbiology, August 2000, p. 3415-3420, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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