This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, B.
Right arrow Articles by Häggblom, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, B.
Right arrow Articles by Häggblom, M. M.
Agricola
Right arrow Articles by Song, B.
Right arrow Articles by Häggblom, M. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2000, p. 3446-3453, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation and Characterization of Diverse Halobenzoate-Degrading Denitrifying Bacteria from Soils and Sediments

Bongkeun Song,dagger Norberto J. Palleroni, and Max M. Häggblom*

Department of Biochemistry and Microbiology and Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8525

Received 20 March 2000/Accepted 31 May 2000

Denitrifying bacteria capable of degrading halobenzoates were isolated from various geographical and ecological sites. The strains were isolated after initial enrichment on one of the monofluoro-, monochloro-, or monobromo-benzoate isomers with nitrate as an electron acceptor, yielding a total of 33 strains isolated from the different halobenzoate-utilizing enrichment cultures. Each isolate could grow on the selected halobenzoate with nitrate as the terminal electron acceptor. The isolates obtained on 2-fluorobenzoate could use 2-fluorobenzoate under both aerobic and denitrifying conditions, but did not degrade other halobenzoates. In contrast, the 4-fluorobenzoate isolates degraded 4-fluorobenzoate under denitrifying conditions only, but utilized 2-fluorobenzoate under both aerobic and denitrifying conditions. The strains isolated on either 3-chlorobenzoate or 3-bromobenzoate could use 3-chlorobenzoate, 3-bromobenzoate, and 2- and 4-fluorobenzoates under denitrifying conditions. The isolates were identified and classified on the basis of 16S rRNA gene sequence analysis and their cellular fatty acid profiles. They were placed in nine genera belonging to either the alpha -, beta -, or gamma -branch of the Proteobacteria, namely, Acidovorax, Azoarcus, Bradyrhizobium, Ochrobactrum, Paracoccus, Pseudomonas, Mesorhizobium, Ensifer, and Thauera. These results indicate that the ability to utilize different halobenzoates under denitrifying conditions is ubiquitously distributed in the Proteobacteria and that these bacteria are widely distributed in soils and sediments.


* Corresponding author. Mailing address: Department of Biochemistry and Microbiology, Cook College, Lipman Hall, Rutgers University, 76 Lipman Dr., New Brunswick, NJ 08901-8525. Phone: (732) 932-9763, ext. 326. Fax: (732) 932-8965. E-mail: haggblom{at}aesop.rutgers.edu.

dagger Present address: Department of Geoscience, Princeton University, Princeton, NJ 08544.


Applied and Environmental Microbiology, August 2000, p. 3446-3453, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Carmona, M., Zamarro, M. T., Blazquez, B., Durante-Rodriguez, G., Juarez, J. F., Valderrama, J. A., Barragan, M. J. L., Garcia, J. L., Diaz, E. (2009). Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View. Microbiol. Mol. Biol. Rev. 73: 71-133 [Abstract] [Full Text]  
  • Mulet, M., Gomila, M., Gruffaz, C., Meyer, J.-M., Palleroni, N. J., Lalucat, J., Garcia-Valdes, E. (2008). Phylogenetic analysis and siderotyping as useful tools in the taxonomy of Pseudomonas stutzeri: description of a novel genomovar. Int. J. Syst. Evol. Microbiol. 58: 2309-2315 [Abstract] [Full Text]  
  • Sallam, A., Steinbuchel, A. (2008). Anaerobic and Aerobic Degradation of Cyanophycin by the Denitrifying Bacterium Pseudomonas alcaligenes Strain DIP1 and Role of Three Other Coisolates in a Mixed Bacterial Consortium. Appl. Environ. Microbiol. 74: 3434-3443 [Abstract] [Full Text]  
  • Kalyuzhnaya, M. G., De Marco, P., Bowerman, S., Pacheco, C. C., Lara, J. C., Lidstrom, M. E., Chistoserdova, L. (2006). Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates.. Int. J. Syst. Evol. Microbiol. 56: 2517-2522 [Abstract] [Full Text]  
  • Song, B., Ward, B. B. (2005). Genetic Diversity of Benzoyl Coenzyme A Reductase Genes Detected in Denitrifying Isolates and Estuarine Sediment Communities. Appl. Environ. Microbiol. 71: 2036-2045 [Abstract] [Full Text]  
  • Danko, A. S., Luo, M., Bagwell, C. E., Brigmon, R. L., Freedman, D. L. (2004). Involvement of Linear Plasmids in Aerobic Biodegradation of Vinyl Chloride. Appl. Environ. Microbiol. 70: 6092-6097 [Abstract] [Full Text]  
  • Pace, M. N., Pace, M. N., Mayes, M. A., Jardine, P. M., Mehlhorn, T. L., Zachara, J. M., Bjornstad, B. N. (2003). Quantifying the Effects of Small-Scale Heterogeneities on Flow and Transport in Undisturbed Cores from the Hanford Formation. Vadose Zone J 2: 664-676 [Abstract] [Full Text]  
  • Willems, A., Fernandez-Lopez, M., Munoz-Adelantado, E., Goris, J., De Vos, P., Martinez-Romero, E., Toro, N., Gillis, M. (2003). Description of new Ensifer strains from nodules and proposal to transfer Ensifer adhaerens Casida 1982 to Sinorhizobium as Sinorhizobium adhaerens comb. nov. Request for an Opinion. Int. J. Syst. Evol. Microbiol. 53: 1207-1217 [Abstract] [Full Text]  
  • Taroncher-Oldenburg, G., Griner, E. M., Francis, C. A., Ward, B. B. (2003). Oligonucleotide Microarray for the Study of Functional Gene Diversity in the Nitrogen Cycle in the Environment. Appl. Environ. Microbiol. 69: 1159-1171 [Abstract] [Full Text]  
  • Egland, P. G., Gibson, J., Harwood, C. S. (2001). Reductive, Coenzyme A-Mediated Pathway for 3-Chlorobenzoate Degradation in the Phototrophic Bacterium Rhodopseudomonas palustris. Appl. Environ. Microbiol. 67: 1396-1399 [Abstract] [Full Text]