Community Structure, Cellular rRNA Content, and Activity of Sulfate-Reducing Bacteria in Marine Arctic Sediments

doi:10.1128/AEM.66.8.3592-3602.2000

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ravenschlag, K.
	Articles by Amann, R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ravenschlag, K.
	Articles by Amann, R.


Agricola

	Articles by Ravenschlag, K.
	Articles by Amann, R.

Previous Article | Next Article

Applied and Environmental Microbiology, August 2000, p. 3592-3602, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Community Structure, Cellular rRNA Content, and Activity of Sulfate-Reducing Bacteria in Marine Arctic Sediments

Katrin Ravenschlag,¹ Kerstin Sahm,¹^,^* Christian Knoblauch,² Bo B. Jørgensen,² and Rudolf Amann¹

Molecular Ecology Group¹ and Department of Biogeochemistry,² Max Planck Institute for Marine Microbiology, 28359 Bremen, Germany

Received 8 March 2000/Accepted 16 May 2000

The community structure of sulfate-reducing bacteria (SRB) of a marine Arctic sediment (Smeerenburgfjorden, Svalbard) wascharacterized by both fluorescence in situ hybridization (FISH)and rRNA slot blot hybridization by using group- and genus-specific16S rRNA-targeted oligonucleotide probes. The SRB community wasdominated by members of the Desulfosarcina-Desulfococcus group.This group accounted for up to 73% of the SRB detected and upto 70% of the SRB rRNA detected. The predominance was shown tobe a common feature for different stations along the coast ofSvalbard. In a top-to-bottom approach we aimed to further resolvethe composition of this large group of SRB by using probes forcultivated genera. While this approach failed, directed cloningof probe-targeted genes encoding 16S rRNA was successful and resultedin sequences which were all affiliated with the Desulfosarcina-Desulfococcusgroup. A group of clone sequences (group SVAL1) most closely relatedto Desulfosarcina variabilis (91.2% sequence similarity) was dominantand was shown to be most abundant in situ, accounting for up to54.8% of the total SRB detected. A comparison of the two methodsused for quantification showed that FISH and rRNA slot blot hybridizationgave comparable results. Furthermore, a combination of the twomethods allowed us to calculate specific cellular rRNA contentswith respect to localization in the sediment profile. The rRNAcontents of Desulfosarcina-Desulfococcus cells were highest inthe first 5 mm of the sediment (0.9 and 1.4 fg, respectively)and decreased steeply with depth, indicating that maximal metabolicactivity occurred close to the surface. Based on SRB cell numbers,cellular sulfate reduction rates were calculated. The rates werehighest in the surface layer (0.14 fmol cell¹ day¹), decreased by a factor of 3 within the first 2 cm, and wererelatively constant in deeperlayers.

^* Corresponding author. Present address: TU Hamburg-Harburg, Technical Microbiology, Denickestr. 15, 21071 Hamburg, Germany.Phone: 49-40-428783964. Fax: 49-40-428782909. E-mail: sahm{at}tu-harburg.de.

Applied and Environmental Microbiology, August 2000, p. 3592-3602, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Villanueva, L., Haveman, S. A., Summers, Z. M., Lovley, D. R. (2008). Quantification of Desulfovibrio vulgaris Dissimilatory Sulfite Reductase Gene Expression during Electron Donor- and Electron Acceptor-Limited Growth. Appl. Environ. Microbiol. 74: 5850-5853 [Abstract] [Full Text]
Losekann, T., Knittel, K., Nadalig, T., Fuchs, B., Niemann, H., Boetius, A., Amann, R. (2007). Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea. Appl. Environ. Microbiol. 73: 3348-3362 [Abstract] [Full Text]
Nakatsu, C. H. (2007). Soil Microbial Community Analysis Using Denaturing Gradient Gel Electrophoresis. Soil Sci. 71: 562-571 [Abstract] [Full Text]
Dar, S. A., Yao, L., van Dongen, U., Kuenen, J. G., Muyzer, G. (2007). Analysis of Diversity and Activity of Sulfate-Reducing Bacterial Communities in Sulfidogenic Bioreactors Using 16S rRNA and dsrB Genes as Molecular Markers. Appl. Environ. Microbiol. 73: 594-604 [Abstract] [Full Text]
Dale, A. W., Regnier, P., Van Cappellen, P. (2006). Bioenergetic Controls on Anaerobic Oxidation of Methane (AOM) in Coastal Marine Sediments: A Theoretical Analysis. ajs 306: 246-294 [Abstract] [Full Text]
Karr, E. A., Sattley, W. M., Rice, M. R., Jung, D. O., Madigan, M. T., Achenbach, L. A. (2005). Diversity and Distribution of Sulfate-Reducing Bacteria in Permanently Frozen Lake Fryxell, McMurdo Dry Valleys, Antarctica. Appl. Environ. Microbiol. 71: 6353-6359 [Abstract] [Full Text]
Treude, T., Knittel, K., Blumenberg, M., Seifert, R., Boetius, A. (2005). Subsurface Microbial Methanotrophic Mats in the Black Sea. Appl. Environ. Microbiol. 71: 6375-6378 [Abstract] [Full Text]
Dar, S. A., Kuenen, J. G., Muyzer, G. (2005). Nested PCR-Denaturing Gradient Gel Electrophoresis Approach To Determine the Diversity of Sulfate-Reducing Bacteria in Complex Microbial Communities. Appl. Environ. Microbiol. 71: 2325-2330 [Abstract] [Full Text]
Gillan, D. C., Danis, B., Pernet, P., Joly, G., Dubois, P. (2005). Structure of Sediment-Associated Microbial Communities along a Heavy-Metal Contamination Gradient in the Marine Environment. Appl. Environ. Microbiol. 71: 679-690 [Abstract] [Full Text]
Koizumi, Y., Kojima, H., Fukui, M. (2004). Dominant Microbial Composition and Its Vertical Distribution in Saline Meromictic Lake Kaiike (Japan) as Revealed by Quantitative Oligonucleotide Probe Membrane Hybridization. Appl. Environ. Microbiol. 70: 4930-4940 [Abstract] [Full Text]
Gros, O., Liberge, M., Heddi, A., Khatchadourian, C., Felbeck, H. (2003). Detection of the Free-Living Forms of Sulfide-Oxidizing Gill Endosymbionts in the Lucinid Habitat (Thalassia testudinum Environment). Appl. Environ. Microbiol. 69: 6264-6267 [Abstract] [Full Text]
Bowman, J. P., McCammon, S. A., Gibson, J. A. E., Robertson, L., Nichols, P. D. (2003). Prokaryotic Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments. Appl. Environ. Microbiol. 69: 2448-2462 [Abstract] [Full Text]
Loy, A., Lehner, A., Lee, N., Adamczyk, J., Meier, H., Ernst, J., Schleifer, K.-H., Wagner, M. (2002). Oligonucleotide Microarray for 16S rRNA Gene-Based Detection of All Recognized Lineages of Sulfate-Reducing Prokaryotes in the Environment. Appl. Environ. Microbiol. 68: 5064-5081 [Abstract] [Full Text]
D'Hondt, S., Rutherford, S., Spivack, A. J. (2002). Metabolic Activity of Subsurface Life in Deep-Sea Sediments. Science 295: 2067-2070 [Abstract] [Full Text]
Orphan, V. J., Hinrichs, K.-U., Ussler, W. III, Paull, C. K., Taylor, L. T., Sylva, S. P., Hayes, J. M., Delong, E. F. (2001). Comparative Analysis of Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in Anoxic Marine Sediments. Appl. Environ. Microbiol. 67: 1922-1934 [Abstract] [Full Text]
Detmers, J., Brüchert, V., Habicht, K. S., Kuever, J. (2001). Diversity of Sulfur Isotope Fractionations by Sulfate-Reducing Prokaryotes. Appl. Environ. Microbiol. 67: 888-894 [Abstract] [Full Text]
Ravenschlag, K., Sahm, K., Amann, R. (2001). Quantitative Molecular Analysis of the Microbial Community in Marine Arctic Sediments (Svalbard). Appl. Environ. Microbiol. 67: 387-395 [Abstract] [Full Text]

J. Bacteriol.	Microbiol. Mol. Biol. Rev.	Eukaryot. Cell	All ASM Journals