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Applied and Environmental Microbiology, August 2000, p. 3628-3631, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Immunomagnetic Separation for Recovery of Cryptosporidium parvum and Giardia duodenalis from High-Iron Matrices

Gary P. Yakub* and Kathleen L. Stadterman-Knauer

Allegheny County Sanitary Authority, Pittsburgh, Pennsylvania 15233

Received 23 December 1999/Accepted 15 May 2000

In this study we examined the recovery of Cryptosporidium parvum and Giardia duodenalis from matrices containing various concentrations of dissolved iron. The organisms were recovered by using the immunomagnetic separation-immunofluorescent assay method, and the levels of recovery were compared to the dissolved iron concentrations. The levels of recovery of C. parvum decreased sharply at dissolved iron concentrations greater than 4 mg/liter, while the levels of recovery of G. duodenalis decreased sharply at concentrations greater than 40 mg/liter.

* Corresponding author. Mailing address: Allegheny County Sanitary Authority, 3300 Preble Avenue, Pittsburgh, PA 15233. Phone: (412) 766-4810. Fax: (412) 732-8023. E-mail: gyakub{at}alcosan.org.

Applied and Environmental Microbiology, August 2000, p. 3628-3631, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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