This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bond, P. L.
Right arrow Articles by Banfield, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bond, P. L.
Right arrow Articles by Banfield, J. F.
Agricola
Right arrow Articles by Bond, P. L.
Right arrow Articles by Banfield, J. F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2000, p. 3842-3849, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Phylogeny of Microorganisms Populating a Thick, Subaerial, Predominantly Lithotrophic Biofilm at an Extreme Acid Mine Drainage Site

Philip L. Bond,* Steven P. Smriga, and Jillian F. Banfield

Department of Geology and Geophysics, University of Wisconsin---Madison, Madison, Wisconsin 53706

Received 14 December 1999/Accepted 6 June 2000

An unusually thick (~1 cm) slime developed on a slump of finely disseminated pyrite ore within an extreme acid mine drainage site at Iron Mountain, near Redding, Calif. The slime was studied over the period of 1 year. The subaerial form of the slime distinguished it from more typical submerged streamers. Phylogenetic analysis of 16S rRNA genes revealed a diversity of sequences that were mostly novel. Nearest relatives to the majority of sequences came from iron-oxidizing acidophiles, and it appears that iron oxidation is the predominant metabolic characteristic of the organisms in the slime. The most abundant of the 16S rRNA genes detected were from organisms related to Leptospirillum species. The dominant sequence (71% of clones) may represent a new genus. Sequences within the Archaea of the Thermoplasmales lineage were detected. Most of these were only distantly related to known microorganisms. Also, sequences affiliating with Acidimicrobium were detected. Some of these were closely related to "Ferromicrobium acidophilus," and others were affiliated with a lineage only represented by environmental clones. Unexpectedly, sequences that affiliated within the delta subdivision of the Proteobacteria were detected. The predominant metabolic feature of bacteria of this subdivision is anaerobic sulfate or metal reduction. Thus, microenvironments of low redox potential possibly exist in the predominantly oxidizing environments of the slime. These results expand our knowledge of the biodiversity of acid mine drainage environments and extend our understanding of the ecology of extremely acidic systems.

* Corresponding author. Mailing address: Department of Geology and Geophysics, University of Wisconsin---Madison, Madison, WI 53706. Phone: (608) 262-0915. Fax: (608) 262-0693. E-mail: bond{at}geology.wisc.edu.

Applied and Environmental Microbiology, September 2000, p. 3842-3849, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Baker, B. J., Tyson, G. W., Goosherst, L., Banfield, J. F. (2009). Insights into the Diversity of Eukaryotes in Acid Mine Drainage Biofilm Communities. Appl. Environ. Microbiol. 75: 2192-2199 [Abstract] [Full Text]  
  • Heinzel, E., Hedrich, S., Janneck, E., Glombitza, F., Seifert, J., Schlomann, M. (2009). Bacterial Diversity in a Mine Water Treatment Plant. Appl. Environ. Microbiol. 75: 858-861 [Abstract] [Full Text]  
  • Singer, S. W., Chan, C. S., Zemla, A., VerBerkmoes, N. C., Hwang, M., Hettich, R. L., Banfield, J. F., Thelen, M. P. (2008). Characterization of Cytochrome 579, an Unusual Cytochrome Isolated from an Iron-Oxidizing Microbial Community. Appl. Environ. Microbiol. 74: 4454-4462 [Abstract] [Full Text]  
  • Salka, I., Moulisova, V., Koblizek, M., Jost, G., Jurgens, K., Labrenz, M. (2008). Abundance, Depth Distribution, and Composition of Aerobic Bacteriochlorophyll a-Producing Bacteria in Four Basins of the Central Baltic Sea. Appl. Environ. Microbiol. 74: 4398-4404 [Abstract] [Full Text]  
  • Mendez, M. O., Neilson, J. W., Maier, R. M. (2008). Characterization of a Bacterial Community in an Abandoned Semiarid Lead-Zinc Mine Tailing Site. Appl. Environ. Microbiol. 74: 3899-3907 [Abstract] [Full Text]  
  • Blothe, M., Akob, D. M., Kostka, J. E., Goschel, K., Drake, H. L., Kusel, K. (2008). pH Gradient-Induced Heterogeneity of Fe(III)-Reducing Microorganisms in Coal Mining-Associated Lake Sediments. Appl. Environ. Microbiol. 74: 1019-1029 [Abstract] [Full Text]  
  • Cleaver, A. A., Burton, N. P., Norris, P. R. (2007). A Novel Acidimicrobium Species in Continuous Cultures of Moderately Thermophilic, Mineral-Sulfide-Oxidizing Acidophiles. Appl. Environ. Microbiol. 73: 4294-4299 [Abstract] [Full Text]  
  • Flanagan, J. L., Brodie, E. L., Weng, L., Lynch, S. V., Garcia, O., Brown, R., Hugenholtz, P., DeSantis, T. Z., Andersen, G. L., Wiener-Kronish, J. P., Bristow, J. (2007). Loss of Bacterial Diversity during Antibiotic Treatment of Intubated Patients Colonized with Pseudomonas aeruginosa. J. Clin. Microbiol. 45: 1954-1962 [Abstract] [Full Text]  
  • Zwolinski, M. D. (2007). DNA Sequencing: Strategies for Soil Microbiology. Soil Sci. 71: 592-600 [Abstract] [Full Text]  
  • Baker, B. J., Tyson, G. W., Webb, R. I., Flanagan, J., Hugenholtz, P., Allen, E. E., Banfield, J. F. (2006). Lineages of Acidophilic Archaea Revealed by Community Genomic Analysis. Science 314: 1933-1935 [Abstract] [Full Text]  
  • Nicomrat, D., Dick, W. A., Tuovinen, O. H. (2006). Microbial populations identified by fluorescence in situ hybridization in a constructed wetland treating Acid coal mine drainage.. J. Environ. Qual. 35: 1329-1337 [Abstract] [Full Text]  
  • Hallberg, K. B., Coupland, K., Kimura, S., Johnson, D. B. (2006). Macroscopic streamer growths in acidic, metal-rich mine waters in north wales consist of novel and remarkably simple bacterial communities.. Appl. Environ. Microbiol. 72: 2022-2030 [Abstract] [Full Text]  
  • Henneberger, R., Moissl, C., Amann, T., Rudolph, C., Huber, R. (2006). New Insights into the Lifestyle of the Cold-Loving SM1 Euryarchaeon: Natural Growth as a Monospecies Biofilm in the Subsurface. Appl. Environ. Microbiol. 72: 192-199 [Abstract] [Full Text]  
  • Bruneel, O., Duran, R., Casiot, C., Elbaz-Poulichet, F., Personne, J.-C. (2006). Diversity of Microorganisms in Fe-As-Rich Acid Mine Drainage Waters of Carnoules, France. Appl. Environ. Microbiol. 72: 551-556 [Abstract] [Full Text]  
  • Tyson, G. W., Lo, I., Baker, B. J., Allen, E. E., Hugenholtz, P., Banfield, J. F. (2005). Genome-Directed Isolation of the Key Nitrogen Fixer Leptospirillum ferrodiazotrophum sp. nov. from an Acidophilic Microbial Community. Appl. Environ. Microbiol. 71: 6319-6324 [Abstract] [Full Text]  
  • Baker-Austin, C., Dopson, M., Wexler, M., Sawers, R. G., Bond, P. L. (2005). Molecular insight into extreme copper resistance in the extremophilic archaeon 'Ferroplasma acidarmanus' Fer1. Microbiology 151: 2637-2646 [Abstract] [Full Text]  
  • Kanugula, S., Pauly, G. T., Moschel, R. C., Pegg, A. E. (2005). A bifunctional DNA repair protein from Ferroplasma acidarmanus exhibits O6-alkylguanine-DNA alkyltransferase and endonuclease V activities. Proc. Natl. Acad. Sci. USA 102: 3617-3622 [Abstract] [Full Text]  
  • Handelsman, J. (2004). Metagenomics: Application of Genomics to Uncultured Microorganisms. Microbiol. Mol. Biol. Rev. 68: 669-685 [Abstract] [Full Text]  
  • Banat, I. M., Marchant, R., Rahman, T. J. (2004). Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.. Int. J. Syst. Evol. Microbiol. 54: 2197-2201 [Abstract] [Full Text]  
  • Baker, B. J., Lutz, M. A., Dawson, S. C., Bond, P. L., Banfield, J. F. (2004). Metabolically Active Eukaryotic Communities in Extremely Acidic Mine Drainage. Appl. Environ. Microbiol. 70: 6264-6271 [Abstract] [Full Text]  
  • Dopson, M., Baker-Austin, C., Hind, A., Bowman, J. P., Bond, P. L. (2004). Characterization of Ferroplasma Isolates and Ferroplasma acidarmanus sp. nov., Extreme Acidophiles from Acid Mine Drainage and Industrial Bioleaching Environments. Appl. Environ. Microbiol. 70: 2079-2088 [Abstract] [Full Text]  
  • Feris, K. P., Ramsey, P. W., Frazar, C., Rillig, M., Moore, J. N., Gannon, J. E., Holben, W. E. (2004). Seasonal Dynamics of Shallow-Hyporheic-Zone Microbial Community Structure along a Heavy-Metal Contamination Gradient. Appl. Environ. Microbiol. 70: 2323-2331 [Abstract] [Full Text]  
  • Baker, B. J., Hugenholtz, P., Dawson, S. C., Banfield, J. F. (2003). Extremely Acidophilic Protists from Acid Mine Drainage Host Rickettsiales-Lineage Endosymbionts That Have Intervening Sequences in Their 16S rRNA Genes. Appl. Environ. Microbiol. 69: 5512-5518 [Abstract] [Full Text]  
  • Feris, K., Ramsey, P., Frazar, C., Moore, J. N., Gannon, J. E., Holben, W. E. (2003). Differences in Hyporheic-Zone Microbial Community Structure along a Heavy-Metal Contamination Gradient. Appl. Environ. Microbiol. 69: 5563-5573 [Abstract] [Full Text]  
  • Gonzalez-Toril, E., Llobet-Brossa, E., Casamayor, E. O., Amann, R., Amils, R. (2003). Microbial Ecology of an Extreme Acidic Environment, the Tinto River. Appl. Environ. Microbiol. 69: 4853-4865 [Abstract] [Full Text]  
  • Dopson, M., Baker-Austin, C., Koppineedi, P. R., Bond, P. L. (2003). Growth in sulfidic mineral environments: metal resistance mechanisms in acidophilic micro-organisms. Microbiology 149: 1959-1970 [Abstract] [Full Text]  
  • Hochella, M. F. Jr (2002). Sustaining Earth: Thoughts on the present and future roles of mineralogy in environmental science. Mineral Mag 66: 627-652 [Abstract] [Full Text]  
  • Newman, D. K., Banfield, J. F. (2002). Geomicrobiology: How Molecular-Scale Interactions Underpin Biogeochemical Systems. Science 296: 1071-1077 [Abstract] [Full Text]  
  • Coram, N. J., Rawlings, D. E. (2002). Molecular Relationship between Two Groups of the Genus Leptospirillum and the Finding that Leptospirillum ferriphilum sp. nov. Dominates South African Commercial Biooxidation Tanks That Operate at 40{degrees}C. Appl. Environ. Microbiol. 68: 838-845 [Abstract] [Full Text]  
  • Bond, P. L., Druschel, G. K., Banfield, J. F. (2000). Comparison of Acid Mine Drainage Microbial Communities in Physically and Geochemically Distinct Ecosystems. Appl. Environ. Microbiol. 66: 4962-4971 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»