AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gobbetti, M.
Right arrow Articles by Addeo, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gobbetti, M.
Right arrow Articles by Addeo, F.
Agricola
Right arrow Articles by Gobbetti, M.
Right arrow Articles by Addeo, F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2000, p. 3898-3904, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Production of Angiotensin-I-Converting-Enzyme-Inhibitory Peptides in Fermented Milks Started by Lactobacillus delbrueckii subsp. bulgaricus SS1 and Lactococcus lactis subsp. cremoris FT4

M. Gobbetti,1,* P. Ferranti,2 E. Smacchi,3 F. Goffredi,3 and F. Addeo4

Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi di Bari, 70126 Bari,1 Centro Internazionale Servizi di Spettrometria di Massa, Area della Ricerca CNR, 80131 Naples,2 Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agro-alimentare, Università degli Studi di Perugia, S. Costanzo, 06126 Perugia,3 and Istituto di Industrie Agrarie, Facoltà di Agraria, Università degli Studi di Napoli, 80055 Portici,4 Italy

Received 7 March 2000/Accepted 30 June 2000

Two fermented milks containing angiotensin-I-converting-enzyme (ACE)-inhibitory peptides were produced by using selected Lactobacillus delbrueckii subsp. bulgaricus SS1 and L. lactis subsp. cremoris FT4. The pH 4.6-soluble nitrogen fraction of the two fermented milks was fractionated by reversed-phase fast-protein liquid chromatography. The fractions which showed the highest ACE-inhibitory indexes were further purified, and the related peptides were sequenced by tandem fast atom bombardment-mass spectrometry. The most inhibitory fractions of the milk fermented by L. delbrueckii subsp. bulgaricus SS1 contained the sequences of beta -casein (beta -CN) fragment 6-14 (f6-14), f7-14, f73-82, f74-82, and f75-82. Those from the milk fermented by L. lactis subsp. cremoris FT4 contained the sequences of beta -CN f7-14, f47-52, and f169-175 and kappa -CN f155-160 and f152-160. Most of these sequences had features in common with other ACE-inhibitory peptides reported in the literature. In particular, the beta -CN f47-52 sequence had high homology with that of angiotensin-II. Some of these peptides were chemically synthesized. The 50% inhibitory concentrations (IC50s) of the crude purified fractions containing the peptide mixture were very low (8.0 to 11.2 mg/liter). When the synthesized peptides were used individually, the ACE-inhibitory activity was confirmed but the IC50s increased considerably. A strengthened inhibitory effect of the peptide mixtures with respect to the activity of individual peptides was presumed. Once generated, the inhibitory peptides were resistant to further proteolysis either during dairy processing or by trypsin and chymotrypsin.

* Corresponding author. Present address: Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agro-alimentare, Università degli Studi di Perugia, S. Costanzo, 06126 Perugia, Italy. Phone: 39 75 32387. Fax: 39 75 32387. E-mail: gobbetti{at}unipg.it.

Applied and Environmental Microbiology, September 2000, p. 3898-3904, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2000 by the American Society for Microbiology. All rights reserved.