This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Choi, S. H.
Right arrow Articles by Kaspar, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choi, S. H.
Right arrow Articles by Kaspar, C. W.
Agricola
Right arrow Articles by Choi, S. H.
Right arrow Articles by Kaspar, C. W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2000, p. 3911-3916, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Contribution of dps to Acid Stress Tolerance and Oxidative Stress Tolerance in Escherichia coli O157:H7

Sang Ho Choi,1,2 David J. Baumler,1 and Charles W. Kaspar1,3,*

Departments of Food Microbiology and Toxicology1 and Animal Sciences,3 Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706-1187, and Department of Food Science and Technology, Institute of Biotechnology, Chonnam National University, Kwang-Ju 500-757, South Korea2

Received 13 March 2000/Accepted 22 June 2000

An Escherichia coli O157:H7 dps::nptI mutant (FRIK 47991) was generated, and its survival was compared to that of the parent in HCl (synthetic gastric fluid, pH 1.8) and hydrogen peroxide (15 mM) challenges. The survival of the mutant in log phase (5-h culture) was significantly impaired (4-log10-CFU/ml reduction) compared to that of the parent strain (ca. 1.0-log10-CFU/ml reduction) after a standard 3-h acid challenge. Early-stationary-phase cells (12-h culture) of the mutant decreased by ca. 4 log10 CFU/ml while the parent strain decreased by approximately 2 log10 CFU/ml. No significant differences in the survival of late-stationary-phase cells (24-h culture) between the parent strain and the mutant were observed, although numbers of the parent strain declined less in the initial 1 h of acid challenge. FRIK 47991 was more sensitive to hydrogen peroxide challenge than was the parent strain, although survival improved in stationary phase. Complementation of the mutant with a functional dps gene restored acid and hydrogen peroxide tolerance to levels equal to or greater than those exhibited by the parent strain. These results demonstrate that decreases in survival were from the absence of Dps or a protein regulated by Dps. The results from this study establish that Dps contributes to acid tolerance in E. coli O157:H7 and confirm the importance of Dps in oxidative stress protection.

* Corresponding author. Mailing address: Food Research Institute, 1925 Willow Dr., University of Wisconsin, Madison, WI 53706-1187. Phone: (608) 263-6936. Fax: (608) 263-1114. E-mail: cwkaspar{at}facstaff.wisc.edu.

Applied and Environmental Microbiology, September 2000, p. 3911-3916, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kailasan Vanaja, S., Bergholz, T. M., Whittam, T. S. (2009). Characterization of the Escherichia coli O157:H7 Sakai GadE Regulon. J. Bacteriol. 191: 1868-1877 [Abstract] [Full Text]  
  • Bore, E., Hebraud, M., Chafsey, I., Chambon, C., Skjaeret, C., Moen, B., Moretro, T., Langsrud, O., Rudi, K., Langsrud, S. (2007). Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses. Microbiology 153: 935-946 [Abstract] [Full Text]  
  • Ceci, P., Mangiarotti, L., Rivetti, C., Chiancone, E. (2007). The neutrophil-activating Dps protein of Helicobacter pylori, HP-NAP, adopts a mechanism different from Escherichia coli Dps to bind and condense DNA. Nucleic Acids Res 35: 2247-2256 [Abstract] [Full Text]  
  • Hung, K. F., Byrd, J. J., Bose, J. L., Kaspar, C. W. (2006). Reduction of Acid Tolerance by Tetracycline in Escherichia coli Expressing tetA(C) Is Reversed by Cations.. Appl. Environ. Microbiol. 72: 4472-4474 [Abstract] [Full Text]  
  • Bearson, S. M. D., Bearson, B. L., Rasmussen, M. A. (2006). Identification of Salmonella enterica Serovar Typhimurium Genes Important for Survival in the Swine Gastric Environment. Appl. Environ. Microbiol. 72: 2829-2836 [Abstract] [Full Text]  
  • Ceci, P., Cellai, S., Falvo, E., Rivetti, C., Rossi, G. L., Chiancone, E. (2004). DNA condensation and self-aggregation of Escherichia coli Dps are coupled phenomena related to the properties of the N-terminus. Nucleic Acids Res 32: 5935-5944 [Abstract] [Full Text]  
  • Naim, F., Messier, S., Saucier, L., Piette, G. (2004). Postprocessing In Vitro Digestion Challenge To Evaluate Survival of Escherichia coli O157:H7 in Fermented Dry Sausages. Appl. Environ. Microbiol. 70: 6637-6642 [Abstract] [Full Text]  
  • Jeong, H. S., Lee, M. H., Lee, K.-H., Park, S.-J., Choi, S. H. (2003). SmcR and Cyclic AMP Receptor Protein Coactivate Vibrio vulnificus vvpE Encoding Elastase through the RpoS-dependent Promoter in a Synergistic Manner. J. Biol. Chem. 278: 45072-45081 [Abstract] [Full Text]  
  • Tucker, D. L., Tucker, N., Conway, T. (2002). Gene Expression Profiling of the pH Response in Escherichia coli. J. Bacteriol. 184: 6551-6558 [Abstract] [Full Text]  
  • Tani, T. H., Khodursky, A., Blumenthal, R. M., Brown, P. O., Matthews, R. G. (2002). Adaptation to famine: A family of stationary-phase genes revealed by microarray analysis. Proc. Natl. Acad. Sci. USA 99: 13471-13476 [Abstract] [Full Text]  
  • Kirkpatrick, C., Maurer, L. M., Oyelakin, N. E., Yoncheva, Y. N., Maurer, R., Slonczewski, J. L. (2001). Acetate and Formate Stress: Opposite Responses in the Proteome of Escherichia coli. J. Bacteriol. 183: 6466-6477 [Abstract] [Full Text]  
  • Jeong, H. S., Jeong, K. C., Choi, H. K., Park, K.-J., Lee, K.-H., Rhee, J. H., Choi, S. H. (2001). Differential Expression of Vibrio vulnificus Elastase Gene in a Growth Phase-dependent Manner by Two Different Types of Promoters. J. Biol. Chem. 276: 13875-13880 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»