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Applied and Environmental Microbiology, September 2000, p. 3917-3923, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Amplified Fragment Length Polymorphism Analysis of Campylobacter jejuni Strains Isolated from Chickens and from Patients with Gastroenteritis or Guillain-Barré or Miller Fisher Syndrome

Birgitta Duim,1,* C. Wim Ang,2 Alex van Belkum,3 Alan Rigter,1 Nan W. J. van Leeuwen,4 Hubert P. Endtz,3 and Jaap A. Wagenaar1

Department of Bacteriology, Institute for Animal Science and Health, Lelystad,1 Departments of Neurology and Immunology2 and Medical Microbiology and Infectious Diseases,3 Erasmus University Center, Rotterdam, and The National Institute of Public Health and the Environment, Bilthoven,4 The Netherlands

Received 29 March 2000/Accepted 6 June 2000

The high-resolution genotyping method of amplified fragment length polymorphism (AFLP) analysis was used to study the genetic relationships between Campylobacter jejuni strains infecting chickens (n = 54) and those causing gastroenteritis in humans (n = 53). In addition, C. jejuni strains associated with the development of Guillain-Barré syndrome (GBS) (n = 14) and Miller Fisher syndrome (MFS) (n = 4), two related acute paralytic syndromes in human, were included. Strains were isolated between 1989 and 1998 in The Netherlands. The AFLP banding patterns were analyzed with correlation-based and band-based similarity coefficients and UPGMA (unweighted pair group method using average linkages) cluster analysis. All C. jejuni strains showed highly heterogeneous fingerprints, and no fingerprints exclusive for chicken strains or for human strains were obtained. All strains were separated in two distinct genetic groups. In group A the percentage of human strains was significantly higher and may be an indication that genotypes of this group are more frequently associated with human diseases. We conclude that C. jejuni from chickens cannot be distinguished from human strains and that GBS or MFS related strains do not belong to a distinct genetic group.

* Corresponding author. Mailing address: Department of Bacteriology, Institute for Animal Science and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Phone: 31-320-238157. Fax: 31-320-238153. E-mail: b.duim{at}id.wag-ur.nl.

Applied and Environmental Microbiology, September 2000, p. 3917-3923, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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