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Applied and Environmental Microbiology, September 2000, p. 4068-4073, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Intracellular Accumulation of Polyphosphate by the Yeast Candida humicola G-1 in Response to Acid pH

John W. McGrath1,2,* and John P. Quinn1,2

School of Biology and Biochemistry1 and QUESTOR Centre,2 The Queen's University of Belfast, Belfast, Northern Ireland

Received 28 February 2000/Accepted 30 May 2000

Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium at pH 5.5 than when grown at pH 7.5. Neither phosphate starvation, nutrient limitation, nor anaerobiosis was required to induce polyP formation. An increase in intracellular polyP was accompanied by a 4.5-fold increase in phosphate uptake from the medium and sixfold-higher levels of cellular polyphosphate kinase activity. This novel accumulation of polyP by C. humicola G-1 in response to acid pH provides further evidence as to the importance of polyP in the physiological adaptation of microbial cells during growth and development and in their response to environmental stresses.

* Corresponding author. Mailing address: School of Biology and Biochemistry, The Queen's University of Belfast, Medical Biology Centre, 97 Lisburn Rd., Belfast, BT9 7BL, Northern Ireland. Phone: (028 90) 245133, ext. 2088 or 2250. Fax: (028 90) 236505. E-mail: j.mcgrath{at}qub.ac.uk.

Applied and Environmental Microbiology, September 2000, p. 4068-4073, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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