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Applied and Environmental Microbiology, September 2000, p. 4112-4114, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Lactococcus lactis as a Cell Factory for High-Level Diacetyl Production

Jeroen Hugenholtz,1,* Michiel Kleerebezem,1 Marjo Starrenburg,1 Jean Delcour,2 Willem de Vos,1 and Pascal Hols1,2,dagger

Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands,1 and Laboratoire de Génétique Moléculaire, Université Catholique de Louvain, B-1348 Louvain-la-Neuve, Belgium2

Received 1 February 2000/Accepted 7 June 2000

We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha -acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha -acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.

* Corresponding author. Mailing address: NIZO Food Research, Department of Flavour and Natural Ingredients, P.O. Box 20, 6710 BA Ede, The Netherlands. Phone: 31-318-659540. Fax: 31-318-650400. E-mail: hugenhol{at}nizo.nl.

dagger Present address: Laboratoire de Génétique Moléculaire, Université Catholique de Louvain, Louvain, Belgium.

Applied and Environmental Microbiology, September 2000, p. 4112-4114, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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