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Applied and Environmental Microbiology, September 2000, p. 4115-4118, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Quantitative Immunocapture PCR Assay for Detection of Campylobacter jejuni in Foods

David F. Waller and Steven A. Ogata*

Hawaii Biotechnology Group, Inc., Aiea, Hawaii

Received 11 February 2000/Accepted 7 June 2000

The rapid detection of food-borne bacterial pathogens as part of a quality control program is necessary for the maintenance of a safe food supply. In this report, we present our findings for an immunocapture PCR method for the detection of Campylobacter jejuni in foods. The method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 8 h. Assay results are quantitative, and one cell in a milliliter sample can be detected. Application of the method to spiked milk samples and chicken skin washes did not affect the sensitivity of the assay.

* Corresponding author. Mailing address: Hawaii Biotechnology Group, Inc., 99-193 Aiea Heights Dr., Suite 236, Aiea, HI 96701. Phone: (808) 486-5333. Fax: (808) 487-7341. E-mail: sogata{at}hibiotech.com.

Applied and Environmental Microbiology, September 2000, p. 4115-4118, Vol. 66, No. 9
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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