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Applied and Environmental Microbiology, September 2000, p. 4124-4127, Vol. 66, No. 9
Roman L. Hruska U.S. Meat Animal Research
Center, Agricultural Research Service, United States Department of
Agriculture, Clay Center, Nebraska 68933
Received 27 March 2000/Accepted 16 June 2000
Monoclonal antibody (MAb) 12F5 reacted with 35 Escherichia
coli O26 isolates and cross-reacted with 1 of 365 non-E.
coli O26 isolates. MAb 15C4 reacted with 30 E. coli
O111 strains and 8 Salmonella O35 strains (possessing
identical O antigen) but not with 362 other bacterial strains.
Lipopolysaccharide immunoblots confirmed MAb O-antigen specificity.
0099-2240/00/$04.00+0
Murine Monoclonal Antibodies Specific for
Lipopolysaccharide of Escherichia coli O26 and
O111
*
Corresponding author. Mailing address: USDA, ARS, U.S.
Meat Animal Research Center, P. O. Box 166, State Spur 18D, Clay
Center, NE 68933. Phone: (402) 762-4343. Fax: (402) 762-4375. E-mail: keen{at}emailmarc.usda.gov.
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