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Applied and Environmental Microbiology, September 2000, p. 4124-4127, Vol. 66, No. 9
0099-2240/00/$04.00+0

Murine Monoclonal Antibodies Specific for Lipopolysaccharide of Escherichia coli O26 and O111

Mildred Rivera-Betancourt and James E. Keen*

Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, United States Department of Agriculture, Clay Center, Nebraska 68933

Received 27 March 2000/Accepted 16 June 2000

Monoclonal antibody (MAb) 12F5 reacted with 35 Escherichia coli O26 isolates and cross-reacted with 1 of 365 non-E. coli O26 isolates. MAb 15C4 reacted with 30 E. coli O111 strains and 8 Salmonella O35 strains (possessing identical O antigen) but not with 362 other bacterial strains. Lipopolysaccharide immunoblots confirmed MAb O-antigen specificity.


* Corresponding author. Mailing address: USDA, ARS, U.S. Meat Animal Research Center, P. O. Box 166, State Spur 18D, Clay Center, NE 68933. Phone: (402) 762-4343. Fax: (402) 762-4375. E-mail: keen{at}emailmarc.usda.gov.


Applied and Environmental Microbiology, September 2000, p. 4124-4127, Vol. 66, No. 9
0099-2240/00/$04.00+0



This article has been cited by other articles:

  • Durso, L. M., Bono, J. L., Keen, J. E. (2005). Molecular Serotyping of Escherichia coli O26:H11. Appl. Environ. Microbiol. 71: 4941-4944 [Abstract] [Full Text]  
  • Rivera-Betancourt, M., Keen, J. E. (2001). Murine Monoclonal Antibodies against Escherichia coli O4 Lipopolysaccharide and H5 Flagellin. J. Clin. Microbiol. 39: 3409-3413 [Abstract] [Full Text]